低温胁迫和赤霉素对花生种子萌发和幼苗生理响应的影响
常博文,钟鹏,刘杰,唐中华,高亚冰,于洪久,郭炜

Effect of low-temperature stress and gibberellin on seed germination and seedling physiological responses in peanut
Bo-Wen CHANG,Peng ZHONG,Jie LIU,Zhong-Hua TANG,Ya-Bing GAO,Hong-Jiu YU,Wei GUO
表4 低温胁迫和GA3对花生幼苗相对膜透性、丙二醛、可溶性糖、可溶性蛋白和游离脯氨酸含量的影响
Table 4 Effects of low-temperature and GA3 on the relative membrane permeability, malondialdehyde (MDA), soluble sugar, soluble protein, and free proline contents of peanut seedlings of different cultivars
生理指标
Physical
indexes
花生品种
Peanut cultivar
对照组
Control group
处理组Treatment group
4℃ 4℃+100
μmol L-1 GA3
4℃+200
μmol L-1 GA3
4℃+300
μmol L-1 GA3
相对膜透性
Relative membrane permeability (%)
阜花17 Fuhua 17 17.10±1.89 ab 24.63±2.08 a 21.63±1.65 a 20.33±1.32 a 18.71±1.16 a
阜花12 Fuhua 12 18.50±1.92 b 26.98±1.36 a 24.56±1.01 b 22.68±1.06 ab 20.22±1.45 ab
冀花16 Jihua16 15.92±1.65 a 26.88±2.02 a 25.69±1.54 b 23.79±0.98 b 21.25±0.99 b
冀花18 Jihua 18 15.32±1.54 a 25.54±1.85 a 24.46±1.99 ab 23.03±1.33 b 21.46±1.25 b
白沙1016 Baisha 1016 16.43±1.98 ab 31.23±1.89 b 30.36±1.45 c 28.46±1.85 c 26.18±1.28 c
鲁花11 Luhua 11 17.45±2.01 ab 31.23±1.97 b 30.56±1.67 c 28.12±1.11 c 26.54±1.98 c
正农黑花生1号
Zhengnongheihuasheng 1
17.95±1.85 ab
30.91±1.16 b
28.63±1.22 c
27.56±1.00 c
25.87±1.18 c
白玉Baiyu 15.99±1.89 a 30.88±1.96 b 29.36±1.02 c 27.98±1.45 c 26.87±1.08 c
丙二醛含量
MDA content
(μmol g-1 FW-1)
阜花17 Fuhua 17 7.13±0.95 a 9.56±1.04 a 8.66±0.83 a 7.84±0.66 a 7.16±0.58 a
阜花12 Fuhua 12 7.56±0.96 a 9.84±0.68 a 8.92±0.51 a 8.06±0.53 a 7.98±0.73 a
冀花16 Jihua 16 6.35±0.83 a 10.19±1.01 a 9.13±0.77 a 8.46±0.49 a 7.39±0.50 a
冀花18 Jihua 18 6.84±0.77 a 10.23±0.93 a 9.04±1.00 a 8.16±0.67 a 7.28±0.63 a
白沙1016 Baisha 1016 6.45±0.99 a 19.03±0.95 b 17.85±0.73 b 16.32±0.93 c 14.88±1.06 c
鲁花11 Luhua 11 7.26±1.01 a 18.32±0.99 b 16.98±0.84 b 15.36±0.56 bc 13.86±0.99 bc
正农黑花生1号
Zhengnongheihuasheng 1
7.06±0.93 a
19.26±0.58 b
16.54±1.05 b
14.46±0.50 b
11.65±1.46 b
白玉 Baiyu 6.98±0.95 a 19.69±0.98 b 17.05±1.51 b 15.24±0.73 bc 12.74±1.22 bc
可溶性糖
Soluble sugar content (mg g-1 FW-1)
阜花17 Fuhua 17 7.46±1.95 b 18.75±1.49 b 21.36±0.83 b 24.68±1.66 bc 29.00±1.28 b
阜花12 Fuhua 12 7.96±1.36 b 18.32±1.38 b 20.69±0.51 b 24.12±1.53 bc 28.56±1.73 b
冀花16 Jihua 16 8.63±1.26 b 17.11±1.51 b 21.63±0.77 b 25.55±1.49 c 27.36±1.00 b
冀花18 Jihua 18 7.78±1.53 b 18.56±1.43 b 20.45±1.00 b 22.69±1.67 b 28.33±1.63 b
白沙1016 Baisha 1016 5.55±0.99 ab 8.78±1.95 a 10.96±0.73 a 12.15±1.90 a 14.15±1.56 a
鲁花11 Luhua 11 4.99±1.01 a 8.82±1.49 a 10.65±0.84 a 12.21±1.56 a 14.54±0.99 a
正农黑花生1号
Zhengnongheihuasheng 1
5.87±0.93 ab
9.74±1.28 a
11.96±1.05 a
13.33±1.51 a
15.78±1.46 a
白玉Baiyu 6.45±1.34 ab 8.64±1.48 a 11.62±1.51 a 13.05±1.73 a 14.51±1.22 a
生理指标
Physical
indexes
花生品种
Peanut cultivar
对照组
Control group
处理组Treatment group
4℃ 4℃+100
μmol L-1 GA3
4℃+200
μmol L-1 GA3
4℃+300
μmol L-1 GA3
可溶性蛋白
Soluble protein content (μmol g-1 FW-1)
阜花17 Fuhua 17 16.49±2.09 a 25.45±0.98 b 26.85±1.65 b 27.96±1.66 b 29.64±1.28 b
阜花12 Fuhua 12 17.68±1.22 a 23.78±0.76 ab 26.45±1.01 c 28.65±1.53 b 30.77±1.73 b
冀花16 Jihua 16 17.45±2.52 a 24.36±1.22 ab 25.85±1.54 bc 26.56±1.49 ab 29.86±1.00 b
冀花18 Jihua 18 16.12±2.06 a 25.88±1.85b 26.74±1.99 b 27.65±1.67 b 30.55±1.03 b
白沙1016 Baisha 1016 18.02±1.98 a 23.71±1.89 ab 24.56±1.45 ab 25.74±1.23 ab 26.16±1.56 a
鲁花11 Luhua 11 17.68±2.01 a 23.52±1.67 ab 23.65±1.67 ab 24.31±1.56 a 26.99±0.99 a
正农黑花生1号
Zhengnongheihuasheng 1
16.59±1.85 a
22.34±1.56 a
23.99±1.35 ab
24.15±1.50 a
26.59±1.46 a
白玉Baiyu 17.66±2.68 a 21.90±1.96 a 23.06±1.02 a 23.94±1.73 a 25.35±1.22 a
游离脯氨酸
Free proline
(μmol g-1 FW-1)
阜花17 Fuhua 17 60.01±7.23 a 83.95±7.24 a 100.65±6.23 ab 112.14±8.66 ab 135.63±7.85 bc
阜花12 Fuhua 12 65.99±7.01 a 85.45±8.36 a 99.46±7.12 ab 116.32±8.12 b 140.63±7.78 c
冀花16 Jihua 16 61.23±8.96 a 86.56±8.11 a 101.25±7.52 ab 110.36±7.78 ab 136.44±8.33 bc
冀花18 Jihua 18 59.46±8.25 a 80.11±8.46 a 103.66±6.66 b 120.56±8.36 b 133.78±7.55 b
白沙1016 Baisha 1016 63.19±4.48 a 78.25±7.12 a 90.26±7.16 a 108.36±7.45 ab 122.32±6.06 ab
鲁花11 Luhua 11 61.85±6.85 a 79.33±7.56 a 90.29±8.56 a 104.85±6.21 a 118.62±7.60 a
正农黑花生1号
Zhengnongheihuasheng 1
58.36±6.99 a
74.63±7.98 a
91.29±7.31 a
102.24±6.65 a
121.55±8.32 ab
白玉 Baiyu 62.45±7.11 a 79.35±8.24 a 95.11±8.56 ab 104.15±7.12 a 119.62±8.41 a