大豆7S球蛋白α'亚基缺失新种质中黄608的分子鉴定

李俊英,孙如建,李忠峰,魏中艳,任玉龙,王俊,邱丽娟

Molecular identification of a new soybean germplasm Zhonghuang 608 lacking of 7S globulin alpha' subunit

Jun-Ying LI,Ru-Jian SUN,Zhong-Feng LI,Zhong-Yan WEI,Yu-Long REN,Jun WANG,Li-Juan QIU

表1 基因Cgy-1 PCR扩增引物

Table 1 Primers for amplifying the Cgy-1 by PCR

引物名称 Name 引物序列 Sequence of primer (5'-3') PCR产物长度 PCR product size (bp) 退火温度 Annealing temperature (℃) Cgy1-A Forward: AGCCCAAAACATTCACCAAC 1598 58 Reverse: AGGACTGTTGAGCTTGAGTGC Cgy1-B Forward: CGCCATACCCGTTAACAAAC 1702 58 Reverse: TTGTGGCAGGACATTGCTAC