大豆7S球蛋白α'亚基缺失新种质中黄608的分子鉴定

李俊英,孙如建,李忠峰,魏中艳,任玉龙,王俊,邱丽娟

Molecular identification of a new soybean germplasm Zhonghuang 608 lacking of 7S globulin alpha' subunit

Jun-Ying LI,Ru-Jian SUN,Zhong-Feng LI,Zhong-Yan WEI,Yu-Long REN,Jun WANG,Li-Juan QIU

表2 dCAPS标记的引物序列和内切酶

Table 2 Primers and restriction endonuclease of the dCAPS marker

标记 Marker 突变位点 SNP 酶 Enzyme 引物序列 Sequence of primer (5'-3') 退火温度 Tm (℃) PCR片段长度 PCR product Size (bp) 酶切后片段长度 Product size after digestion by Taq I GM7S-1 G/A Taq I F: TCTCATTTGGCATTGCGTATCG R: TTGACCTTCTTCGCATTCT 55 158 158 bp/(138 bp+20 bp)