大豆7S球蛋白α'亚基缺失新种质中黄608的分子鉴定

李俊英,孙如建,李忠峰,魏中艳,任玉龙,王俊,邱丽娟

Molecular identification of a new soybean germplasm Zhonghuang 608 lacking of 7S globulin alpha' subunit

Jun-Ying LI,Ru-Jian SUN,Zhong-Feng LI,Zhong-Yan WEI,Yu-Long REN,Jun WANG,Li-Juan QIU

图2 α'亚基缺失突变体的基因定位 a: 用210个F2单株将候选基因Cgy-1定位到标记SSR10-0982与SSR10-1638之间。b: 通过多态性标记筛选和加密, 将候选基因定位区间缩小至SSR10-1489与SSR10-1619之间。c: 突变体中黄608在Cgy-1基因第1外显子的第84位碱基发生突变(核苷酸G变为A), 造成编码氨基酸序列的提前终止。蓝色方框代表外显子, 白色方框代表5'和3'UTR, 黑线代表基因的内含子。

Fig. 2 Genetic and physical mapping of the α' subunit deletion mutant Zhonghuang 608 a: the candidate gene Cgy-1 was mapped to between SSR marker SSR10-0982 and SSR10-1638 by using 210 F2 individuals. b: the candidate interval was reduced to SSR10-1489 and SSR10-1619 by SSR polymorphic markers. c: the mutation of eighty-fourth bases at the first exon of Cgy-1 gene in the mutant Zhonghuang 608, resulted in pre-terminating of encoding amino acid. Blue boxes, white boxes, and black lines represent exons, 5' UTR/3' UTR and introns, respectively.