大豆7S球蛋白α'亚基缺失新种质中黄608的分子鉴定

李俊英,孙如建,李忠峰,魏中艳,任玉龙,王俊,邱丽娟

Molecular identification of a new soybean germplasm Zhonghuang 608 lacking of 7S globulin alpha' subunit

Jun-Ying LI,Ru-Jian SUN,Zhong-Feng LI,Zhong-Yan WEI,Yu-Long REN,Jun WANG,Li-Juan QIU

图4 dCAPS分子标记开发 a: 引物GM7S-1分别扩增野生型及突变体, PCR产物为150 bp的单一条带。b: ZH608的扩增产物被Taq I酶酶切为2条更小的片段。

Fig. 4 A agarose detection for amplified products and enzyme products a: the single fragment was detected for the amplified PCR product of both the wild type and the mutant by the primer GM7S-1. b: the PCR product of ZH608 was cleaved into two smaller fragments by the restriction enzyme Taq I.