甘蔗NAD(P)H脱氢酶复合体O亚基基因克隆及其与甘蔗花叶病毒VPg互作

翟玉山,赵贺,张海,邓宇晴,程光远,杨宗桃,王彤,彭磊,徐倩,董萌,徐景升

Cloning of NAD(P)H complex O subunit gene and its interaction with VPg of Sugarcane mosaic virus

Yu-Shan ZHAI,He ZHAO,Hai ZHANG,Yu-Qing DENG,Guang-Yuan CHENG,Zong-Tao YANG,Tong WANG,Lei PENG,Qian XU,Meng DONG,Jing-Sheng XU

表1 本研究使用的引物

Table 1 Primers used in this study

引物名称 Primer name 引物序列 Primer sequence (5′-3′) 用途 Strategy ScNdhO-F ACCAAGCCGAGCAATCCCTC 基因克隆 ScNdhO-R CACCGTGTCCTGACCCCAAG Gene cloning ScNdhO-AD-F GGAATTCCATATGATGGAAGCCCTCGCCTCG 酵母双杂交捕获载体构建 ScNdhO-AD-R CGGAATTCTATCCTCTCGTAGTCGAGCTTG Vector generation for Y2H ScNdhO-YC-F GGGGACAAGTTTGTACAAAAAAGCAGGCTTCATGGAAGCCCTCGCCTCG 双分子荧光互补载体构建 ScNdhO-YC-R GGGGACCACTTTGTACAAGAAAGCTGGGTCTATCCTCTCGTAGTCGAGCTTG Vector generation for BiFC ScNdhO-qF CGATGAAGAAAGGGCAGA 定量PCR ScNdhO-qR TGTAGAATGGCGGGTGTC Real-time-qPCR GAPDH-F CACGGCCACTGGAAGCA 内参基因 GAPDH-R TCCTCAG GGTTCCTGATGCC Reference gene eEF-1α-F TTTCACACTTGGAGTGAAGCAGAT 内参基因 eEF-1α-R GACTTCCTTCACAATCTCATCATAA Reference gene SCMV-CP-F TACAGAGAGACACACAGCTG SCMV检测 SCMV-CP-R ACGCTACACCAGAAGACACT Detection of SCMV