甘蔗NAD(P)H脱氢酶复合体O亚基基因克隆及其与甘蔗花叶病毒VPg互作

翟玉山,赵贺,张海,邓宇晴,程光远,杨宗桃,王彤,彭磊,徐倩,董萌,徐景升

Cloning of NAD(P)H complex O subunit gene and its interaction with VPg of Sugarcane mosaic virus

Yu-Shan ZHAI,He ZHAO,Hai ZHANG,Yu-Qing DENG,Guang-Yuan CHENG,Zong-Tao YANG,Tong WANG,Lei PENG,Qian XU,Meng DONG,Jing-Sheng XU

图5 Y2H检测ScNdhO与SCMV-VPg的互作 pGADT7-T和pGBKT7-53组合作为阳性对照, pGADT7-T和pGBKT7-Lam组合作为阴性对照。SD/-Leu/-Trp: 缺少亮氨酸(Leu)和色氨酸(Trp)的酵母合成限定基本培养基; SD/-Leu/-Trp/-His/-Ade: 缺少亮氨酸(Leu)、色氨酸(Trp)、组氨酸(His)和腺嘌呤(Ade)的酵母合成限定基本培养基。

Fig. 5 Y2H assay for protein-protein interactions between ScNdhO and SCMV-VPg The positive and negative controls are yeast cotransformants with pGADT7-T plus pGBKT7-53 and pGADT7-T plus pGBKT7-Lam, respectively. SD/-Leu/-Trp: synthetic defined yeast minimal medium lacking Leu and Trp; SD/-Leu/-Trp/-His/-Ade: synthetic defined yeast minimal medium lacking Leu, Trp, His, and Ade.