甘蔗NAD(P)H脱氢酶复合体O亚基基因克隆及其与甘蔗花叶病毒VPg互作

翟玉山,赵贺,张海,邓宇晴,程光远,杨宗桃,王彤,彭磊,徐倩,董萌,徐景升

Cloning of NAD(P)H complex O subunit gene and its interaction with VPg of Sugarcane mosaic virus

Yu-Shan ZHAI,He ZHAO,Hai ZHANG,Yu-Qing DENG,Guang-Yuan CHENG,Zong-Tao YANG,Tong WANG,Lei PENG,Qian XU,Meng DONG,Jing-Sheng XU

图6 BiFC检测ScNdhO与SCMV-VPg的互作 ScNdhO融合于YFP的C末端, SCMV-VPg融合于YFP的N末端, 然后在本氏烟叶片中瞬时表达, 48 h后激光共聚焦观察。bar = 50 μm。

Fig. 6 BiFC assay for protein-protein interactions between ScNdhO and VPg from SCMV ScNdhO was fused to the C-terminal half of YFP, while SCMV-VPg was fused to the N-terminal half of YFP. ScNdhO-YC and SCMV-VPg-YN were transiently coexpressed in N. benthamiana leave. The fluorescent signal was monitored by confocal microscopy at 48 hpi. bar = 50 μm.