新疆、甘肃黍稷资源的遗传多样性与群体遗传结构研究

薛延桃,陆平,史梦莎,孙昊月,刘敏轩,王瑞云

Genetic diversity and population genetic structure of broomcorn millet accessions in Xinjiang and Gansu

Yan-Tao XUE,Ping LU,Meng-Sha SHI,Hao-Yue SUN,Min-Xuan LIU,Rui-Yun WANG

表 2 103对SSR标记的遗传参数

Table 2 Genetic parameters from the 103 polymorphic SSR markers used in this study

位点 Locus 观测等位基因 Na 有效等位基因 Ne 多样性指数 I 观测杂合度 Ho 期望杂合度 He Nei’s期望杂 合度Nei 多态性信息含量指数 PIC 近交系数 FIS 遗传分化系数 FST BM5 2 1.9383 0.6772 0.8153 0.5144 0.4841 0.6726 0.6056 0.0011 BM114 2 1.8462 0.6509 0.2990 0.5405 0.4583 0.4586 -0.5072 0.0010 BM136 3 1.5378 0.6510 0.7750 0.6494 0.3497 0.5036 0.4893 0.0605 BM289 3 1.6935 0.6233 0.4865 0.5894 0.4095 0.5562 -0.2865 0.0072 BM295 3 1.6801 0.6751 0.6458 0.5942 0.4048 0.5802 0.0999 0.0248 BM306 3 1.9004 0.8133 0.8699 0.5243 0.4738 0.6085 0.7119 0.0177 BM344 2 1.9130 0.6702 0.9052 0.5216 0.4773 0.5025 0.8210 0.0060 BM374 3 2.0044 0.7710 0.9948 0.4976 0.5011 0.5255 0.9911 0.0555 BM378 4 3.1204 1.1785 0.5956 0.3180 0.6795 0.6992 0.3691 0.0330 BM396 5 3.8669 1.4184 0.4412 0.2568 0.7414 0.6216 0.1709 0.0252 位点 Locus 观测等位基因 Na 有效等位基因 Ne 多样性指数 I 观测杂合度 Ho 期望杂合度 He Nei’s期望 杂合度Nei 多态性信息 含量指数 PIC 近交系数 FIS 遗传分化系数 FST BM484 2 1.9989 0.6929 0.0330 0.4991 0.4997 0.0961 -0.9439 0.0001 BM552 2 1.4272 0.4763 0.9193 0.6998 0.2993 0.5298 0.7391 0.0001 BM637 3 1.9772 0.8574 0.9840 0.5045 0.4942 0.5729 0.9651 0.0194 BM787 2 1.6923 0.5993 0.9949 0.5899 0.4091 0.4409 0.9896 0.0212 BM994 1 1.0000 0.0000 1.0000 1.0000 0.0000 0.1345 — 0.0000 BM1303 3 2.3398 0.9215 0.1317 0.4260 0.5726 0.2976 -0.5275 0.0004 BM1332 3 2.2217 0.8677 0.1055 0.4487 0.5499 0.2960 -0.6839 0.0055 BM1419 3 2.0267 0.7383 0.1078 0.4922 0.5066 0.2653 -0.7938 0.0068 BM1477 4 1.3645 0.4784 1.0000 0.7322 0.2671 0.3471 1.0000 0.0396 BM1533 3 2.0163 0.7229 0.0632 0.4945 0.5040 0.3449 -0.8610 0.0008 BM1745 3 1.5077 0.5428 0.9888 0.6623 0.3368 0.4730 0.9449 0.0513 BM4724 2 1.4196 0.4718 0.7067 0.7037 0.2956 0.4283 -0.0304 0.0696 BM4739 1 1.0000 0.0000 1.0000 1.0000 0.0000 0.0688 — 0.0000 BM4741 2 1.9982 0.6927 0.0299 0.4992 0.4996 0.1703 -0.9493 0.0001 BM4749 2 1.9918 0.6911 0.0640 0.5008 0.4979 0.2091 -0.8783 0.0000 BM4761 2 1.9527 0.6810 0.1651 0.5110 0.4879 0.2696 -0.7295 0.0009 BM4830 2 1.9998 0.6931 0.0488 0.4988 0.5000 0.1752 -0.9021 0.0013 BM4846 2 2.0000 0.6931 0.0485 0.4985 0.5000 0.3555 -0.9108 0.0007 BM4847 3 1.2757 0.3930 1.0000 0.7833 0.2161 0.3205 1.0000 0.0018 BM4851 2 1.9979 0.6926 0.0328 0.4992 0.4995 0.2720 -0.9501 0.0007 BM4858 3 2.8140 1.0671 0.4545 0.3537 0.6446 0.6007 0.0291 0.0539 BM4871 2 1.8181 0.6422 0.3163 0.5489 0.4500 0.4527 -0.5962 0.0189 BM4877 2 2.0000 0.6931 0.0203 0.4987 0.5000 0.1811 -0.9549 0.0005 BM4882 2 1.7734 0.6278 0.3575 0.5628 0.4361 0.4110 -0.5323 0.0131 BM4947 3 2.0330 0.7408 0.0707 0.4906 0.5081 0.2495 -0.8285 0.0011 BM4954 2 1.9966 0.6923 0.0518 0.4996 0.4991 0.2503 -0.8918 0.0010 BM4956 5 3.5531 1.4317 0.7083 0.2793 0.7186 0.7786 0.5725 0.0603 BM4958 4 2.3304 0.9634 0.2062 0.4276 0.5709 0.4514 -0.4649 0.0082 BM4962 3 2.8938 1.0796 0.5833 0.3437 0.6544 0.6773 0.3670 0.0506 BM4965 2 1.6047 0.5644 0.4963 0.6218 0.3768 0.5900 -0.3359 0.0002 BM4967 3 2.5341 0.9923 0.9597 0.3926 0.6054 0.6822 0.9010 0.3009 BM4969 2 1.4404 0.4838 0.7055 0.6932 0.3058 0.5857 0.1076 0.0180 BM4973 2 1.7302 0.6130 0.3949 0.5769 0.4220 0.4811 -0.4310 0.0001 BM4997 4 1.8617 0.8692 0.8986 0.5360 0.4628 0.5248 0.7613 0.0314 BM5037 3 2.3310 0.9322 0.9845 0.4275 0.5710 0.6071 0.9675 0.0392 BM5038 4 2.4239 0.9921 0.9904 0.4112 0.5874 0.5465 0.9764 0.0497 BM5043 3 1.3924 0.4768 0.9158 0.7174 0.2818 0.4556 0.6614 0.0012 BM5181 5 4.4081 1.5426 0.9860 0.2241 0.7731 0.7603 0.9794 0.0273 BM5190 2 1.9877 0.6901 0.0890 0.5018 0.4969 0.3075 -0.8523 0.0008 BM5191 2 1.0649 0.1398 0.9510 0.9388 0.0610 0.4038 0.0714 0.0075 位点 Locus 观测等位基因 Na 有效等位基因 Ne 多样性指数 I 观测杂合度 Ho 期望杂合度 He Nei’s期望 杂合度Nei 多态性信息 含量指数 PIC 近交系数 FIS 遗传分化系数 FST BM5197 3 2.2495 0.9122 0.7278 0.4428 0.5555 0.7230 0.4842 0.0136 BM5198 4 2.3177 1.0676 0.7933 0.4301 0.5685 0.6514 0.6064 0.0402 BM5199 3 1.4330 0.5004 0.9303 0.6971 0.3022 0.4156 0.7935 0.0415 BM5200 3 2.7119 1.0473 0.9310 0.3666 0.6312 0.7036 0.8719 0.0906 BM5222 2 1.0960 0.1862 0.9855 0.9122 0.0876 0.1679 0.8169 0.0739 BM5233 3 2.3928 0.9509 0.8513 0.4164 0.5821 0.6892 0.6708 0.0568 BM5236 5 3.2999 1.3375 0.9838 0.3011 0.6970 0.7312 0.9753 0.0320 BM5255 4 2.7716 1.1500 0.9497 0.3588 0.6392 0.7069 0.9107 0.0090 BM5259 2 1.7637 0.6246 0.9150 0.5656 0.4330 0.6083 0.7945 0.0066 BM5260 4 3.1804 1.2490 0.9110 0.3126 0.6856 0.7343 0.8789 0.0037 BM5278 2 1.8589 0.6547 0.2959 0.5368 0.4620 0.4528 -0.5496 0.0017 BM5279 5 2.0148 0.8350 0.4183 0.4947 0.5037 0.6353 -0.1277 0.0191 BM5292 2 1.8111 0.6400 0.3478 0.5508 0.4478 0.5679 -0.6191 0.1899 LMX258 3 2.1885 0.8523 0.1667 0.4556 0.5431 0.3502 -0.5358 0.0006 LMX503 4 3.0753 1.1609 0.3370 0.3233 0.6748 0.5510 0.0283 0.0609 LMX515 3 1.5659 0.6700 0.8039 0.6377 0.3614 0.3719 0.4715 0.0008 LMX621 4 1.3985 0.5747 1.0000 0.7143 0.2849 0.3751 1.0000 0.0184 LMX632 3 2.0696 0.7690 0.0643 0.4817 0.5168 0.3580 -0.8255 0.0015 LMX635 1 1.0000 0.0000 1.0000 1.0000 0.0000 0.3182 — 0.0000 LMX645 4 2.8467 1.1221 0.2341 0.3497 0.6487 0.4002 -0.3148 0.0415 LMX653 2 1.9231 0.6730 0.2000 0.5184 0.4800 0.5007 -0.6887 0.0056 LMX684 2 1.8824 0.6616 0.2722 0.5299 0.4688 0.4990 -0.5866 0.0099 LMX786 2 1.5437 0.5370 0.6602 0.6469 0.3522 0.4864 -0.3160 0.1710 LMX691 2 1.8243 0.6442 0.3103 0.5470 0.4518 0.4161 -0.5112 0.0090 LMX836 4 1.5324 0.7176 0.9902 0.6517 0.3474 0.4054 0.9634 0.0443 LMX1067 5 1.8316 0.8386 0.8045 0.5447 0.4540 0.5499 0.5335 0.1305 LMX1080 5 3.4824 1.3477 0.4615 0.2850 0.7128 0.6346 0.2112 0.0044 LMX1220 4 1.3272 0.5161 0.9341 0.7528 0.2465 0.4095 0.7219 0.0555 LMX1251 4 2.5935 1.0376 0.3838 0.3840 0.6144 0.5778 -0.2001 0.0480 LMX1380 2 1.9652 0.6843 0.8032 0.5075 0.4912 0.6504 0.5540 0.2873 LMX1387 2 1.9350 0.6763 0.9895 0.5155 0.4832 0.5116 0.9653 0.3534 LMX1400 3 1.7831 0.7811 0.6970 0.5597 0.4392 0.5739 0.1793 0.0256 LMX1429 2 1.1216 0.2200 0.9950 0.8913 0.1084 0.2220 0.9556 0.0270 LMX1625 4 1.9275 0.8626 0.7027 0.5175 0.4812 0.5069 0.3871 0.0083 LMX1672 2 1.9549 0.6816 0.1899 0.5100 0.4885 0.4969 -0.6730 0.0031 LMX1760 4 1.1975 0.3842 0.9780 0.8347 0.1649 0.3505 0.8739 0.0311 LMX1749 4 2.2324 0.9120 1.0000 0.4465 0.5521 0.5729 1.0000 0.2580 LMX1761 4 1.3168 0.5155 0.8987 0.7586 0.2406 0.4564 0.5788 0.0286 LMX1959 3 2.2523 0.8898 0.9875 0.4422 0.5560 0.6362 0.9795 0.0156 LMX2175 4 3.4687 1.3006 0.6364 0.2866 0.7117 0.7296 0.4332 0.0015 LMX2410 2 1.9766 0.6872 0.1088 0.5046 0.4941 0.3185 -0.8091 0.0001 LMX1940 3 1.2271 0.3713 1.0000 0.8145 0.1851 0.2953 1.0000 0.0484 LMX2019 3 2.0034 0.7652 0.2069 0.4905 0.5082 0.3828 -0.6153 0.0087 LMX2068 4 2.6397 1.0942 1.0000 0.3773 0.6212 0.6074 1.0000 0.0130 LMX2074 2 1.0113 0.0347 0.9888 0.9888 0.0112 0.2638 -0.0076 0.0010 LMX2185 3 2.2892 0.9545 0.9899 0.4354 0.5632 0.5831 0.9854 0.0150 LMX2281 2 1.3303 0.4144 1.0000 0.7510 0.2483 0.4096 1.0000 0.0017 LMX2305 4 3.1052 1.1747 0.7943 0.3196 0.6780 0.7425 0.5881 0.1369 LMX2370 2 1.6067 0.5652 0.4947 0.6214 0.3776 0.5126 -0.3722 0.0079 位点 Locus 观测等位基因 Na 有效等位基因 Ne 多样性指数 I 观测杂合度 Ho 期望杂合度 He Nei’s期望 杂合度Nei 多态性信息 含量指数 PIC 近交系数 FIS 遗传分化系数 FST LMX2382 2 1.0937 0.1830 0.9655 0.9141 0.0856 0.4081 0.6754 0.0047 LMX2540 2 1.2207 0.3262 0.8093 0.8187 0.1808 0.3966 -0.0631 0.0030 LMX2734 4 3.0566 1.1767 0.7297 0.3241 0.6728 0.6607 0.5920 0.0151 LMX2782 3 1.4680 0.6063 0.8426 0.6804 0.3188 0.4834 0.5870 0.0185 Mean 2.9029 2.0087 0.7360 0.6298 0.5497 0.4491 0.4714 0.5870 0.0383 SD 0.9952 0.6657 0.3137 0.3517 0.1778 0.1773