基于优化sgRNA系统提高海岛棉CRISPR/Cas9基因组编辑功效的研究
李继洋,胡燕,姚瑞,代培红,刘晓东

Enhancing CRISPR/Cas9 genomic editing efficiency based on optimization of sgRNA of Gossypium barbadense L.
Ji-Yang LI,Yan HU,Rui YAO,Pei-Hong DAI,Xiao-Dong LIU
表2 脱靶序列预测信息
Table 2 Information of off target sequence
脱靶序列名称
Name for sequence of off target
序列
Sequence (5°-3°)
预测脱靶率
Predict the off target rate
OT-GGB-sgRNA1-1 GGTCCAAGGACTGAAGCTGTGG 0.311
OT-GGB-sgRNA1-2 GGTTGAAGTAGTAAAGCGGAGG 0.177
OT-Shift GGB-sgRNA1-1 TCCAGTCGAAATACTGAAGAGG 0.408
OT-Shift GGB-sgRNA1-2 CTCTGTTGATGAACTGATGGGG 0.287