基于优化sgRNA系统提高海岛棉CRISPR/Cas9基因组编辑功效的研究
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李继洋,胡燕,姚瑞,代培红,刘晓东
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Enhancing CRISPR/Cas9 genomic editing efficiency based on optimization of sgRNA of Gossypium barbadense L.
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Ji-Yang LI,Yan HU,Rui YAO,Pei-Hong DAI,Xiao-Dong LIU
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表3 脱靶序列检测引物信息
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Table 3 Information of off target sequence primer
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序列名称
Sequence name | 序列
Sequence (5°-3°) | | OTtest-G1-1F | CCTTGAAAGTTGCTTCCGAC | | OTtest-G1-1R | GAAACTGCCTTTTTTGACACC | | OTtest-G1-2F | CGAAAAATGGTTAGTGGTGAC | | OTtest-G1-2R | GTGGATGTTACTGTAGCAATG | | OTtest-SG1-1F | AGAGGACCAAACACATTAACC | | OTtest-SG1-1R | TTTGGGGGAGAGCACTTTTG | | OTtest-SG1-2F | ATCACCTTGCTACTCTTTCTG | | OTtest-SG1-2R | GTTTAGTTGACCAAGCATCTC |
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