基于优化sgRNA系统提高海岛棉CRISPR/Cas9基因组编辑功效的研究

李继洋,胡燕,姚瑞,代培红,刘晓东

Enhancing CRISPR/Cas9 genomic editing efficiency based on optimization of sgRNA of Gossypium barbadense L.

Ji-Yang LI,Yan HU,Rui YAO,Pei-Hong DAI,Xiao-Dong LIU

表4 转化不同靶序列编辑效率统计结果

Table 4 Statistical results of editing efficiency of different target sequences

转化载体名称 Conversion carrier name 一个位点碱基突变个数 A base muta- tion number 2个位点同时突变个数 Number of mutations for two sites at the same time 突变率 Mutation rate sgRNA1位点 sgRNA1 site sgRNA2位点 sgRNA2 site sgRNA1位点sgRNA1 site sgRNA2位点sgRNA2 site GGB-sgRNA1 12/95 — — 0.126 — Shift GGB-sgRNA1 10/95 — — 0.105 — GGB-sgRNA1-ERA1-sgRNA2 — 15/70 15/70 0.214 0.214 Shift GGB-sgRNA1-ERA1-sgRNA2 — 11/70 10/70 0.157 0.143 GGB-sgRNA1-GGB-sgRNA2 — 9/57 7/57 0.157 0.123 Shift GGB-sgRNA1-GGB-sgRNA2 — 7/60 5/60 0.117 0.083