基于优化sgRNA系统提高海岛棉CRISPR/Cas9基因组编辑功效的研究

李继洋,胡燕,姚瑞,代培红,刘晓东

Enhancing CRISPR/Cas9 genomic editing efficiency based on optimization of sgRNA of Gossypium barbadense L.

Ji-Yang LI,Yan HU,Rui YAO,Pei-Hong DAI,Xiao-Dong LIU

表5 转化不同靶序列脱靶效率统计结果

Table 5 Statistics of off-target efficiency of different target sequences

转化载体名称 Conversion carrier name 脱靶序列检测Detection of off-target sequences OTtest-G1-1 OTtest-SG1-1 OTtest-SG1-2 GGB-sgRNA1 6/50 — — Shift GGB-sgRNA1 — 0 18/50 GGB-sgRNA1-ERA1-sgRNA2 0 — — Shift GGB-sgRNA1-ERA1-sgRNA2 — 0 6/50 GGB-sgRNA1-GGB-sgRNA2 0 — — Shift GGB-sgRNA1-GGB-sgRNA2 — 0 6/50