基于优化sgRNA系统提高海岛棉CRISPR/Cas9基因组编辑功效的研究

李继洋,胡燕,姚瑞,代培红,刘晓东

Enhancing CRISPR/Cas9 genomic editing efficiency based on optimization of sgRNA of Gossypium barbadense L.

Ji-Yang LI,Yan HU,Rui YAO,Pei-Hong DAI,Xiao-Dong LIU

图11 海岛棉脱靶目标序列测序峰图 A、B、C分别为OTtest-SG1-2、OTtest-G1-2、OTtest-SG1-1引物扩增转化下图对应载体得到的目标片段测序结果。红框标识为碱基突变位点。

Fig. 11 Off target sequence sequencing peak map of Gossypium barbadense L. A, B, and C are OTtest-SG1-2, OTtest-G1-2, and OTtest-SG1-1 primers to transform the sequencing result of the target fragment amplified by the corresponding vector in the figure below, respectively. The red box identifies the base mutation site.