基于优化sgRNA系统提高海岛棉CRISPR/Cas9基因组编辑功效的研究

李继洋,胡燕,姚瑞,代培红,刘晓东

Enhancing CRISPR/Cas9 genomic editing efficiency based on optimization of sgRNA of Gossypium barbadense L.

Ji-Yang LI,Yan HU,Rui YAO,Pei-Hong DAI,Xiao-Dong LIU

图3 海岛棉编辑载体酶切检测结果 A: GbU6-5P-GGB-sgRNA1编辑载体酶切检测结果; M1为1 kb plus marker。B: 1和2分别为GbU6-5P::GGB-sgRNA1-GbU6- 4P::GGB-sgRNA2和GbU6-5P::GGB-sgRNA1-GbU6-4P::ERA1- sgRNA2编辑载体酶切检测结果; M2为2 kb plus II marker。

Fig. 3 Result of gene editing vector restriction enzyme digestion of Gossypium barbadense L. A: GbU6-5P-GGB-sgRNA 1 editing vector digestion test results; M1 is 1 kb plus marker. B: GbU6-5P::GGB-sgRNA1-GbU6- 4P::GGB-sgRNA2 and GbU6-5P::GGB-sgRNA1-GbU6-4P::ERA1- sgRNA2 edit vector restriction test results; M2 is 2 kb plus II marker.