基于优化sgRNA系统提高海岛棉CRISPR/Cas9基因组编辑功效的研究

李继洋,胡燕,姚瑞,代培红,刘晓东

Enhancing CRISPR/Cas9 genomic editing efficiency based on optimization of sgRNA of Gossypium barbadense L.

Ji-Yang LI,Yan HU,Rui YAO,Pei-Hong DAI,Xiao-Dong LIU

图4 海岛棉原生质体制备及其转化结果 A: 海岛棉转化前原生质体镜检结果; B: 海岛棉转化GbU6-5P::GGB-sgRNA1-GbU6-4P::GGB-sgRNA2-Cas9I载体后原生质体镜检结果。标尺为40 μm。

Fig. 4 Protoplast preparation and transformation results of Gossypium barbadense L. A: microscopic examination of Gossypium barbadense L. protoplast before transformation; B: after protoplast microscopy results transformation of G. barbadense GbU6-5P::GGB-sgRNA1-GbU6-4P::GGB-sgRNA2-Cas9I vector. Bar = 40 μm.