基于优化sgRNA系统提高海岛棉CRISPR/Cas9基因组编辑功效的研究

李继洋,胡燕,姚瑞,代培红,刘晓东

Enhancing CRISPR/Cas9 genomic editing efficiency based on optimization of sgRNA of Gossypium barbadense L.

Ji-Yang LI,Yan HU,Rui YAO,Pei-Hong DAI,Xiao-Dong LIU

图7 海岛棉转化不同类型靶序列碱基突变部分测序峰图 A: 转化GbU6-5P-GGB-sgRNA1-Cas9I靶序列基因组碱基突变测序峰图; B: 转化GbU6-5P::GGB-sgRNA1-GbU6-4P::ERA1- sgRNA2-Cas9I靶序列基因组碱基突变测序峰图; C: 转化GbU6-5P::GGB-sgRNA1-GbU6-4P::GGB-sgRNA2-Cas9I靶序列基因组碱基突变测序峰图。

Fig. 7 Sequencing peak map of base mutation of different types of target sequences of Gossypium barbadense L. A: baseline mutation map of genomes transformed with GbU6-5P-GGB-sgRNA1-Cas9I target sequence; B: transformation of GbU6- 5P::GGB-sgRNA1-GbU6-4P::ERA1-sgRNA2-Cas9I target sequence; C: baseline mutation sequencing of the genome of transformed GbU6- 5P::GGB-sgRNA1-GbU6-4P::GGB-sgRNA2-Cas9I target sequence.