花生FAD2基因家族表达分析及其对低温胁迫的响应

薛晓梦,李建国,白冬梅,晏立英,万丽云,康彦平,淮东欣,雷永,廖伯寿

Expression profiles of FAD2 genes and their responses to cold stress in peanut

Xiao-Meng XUE,Jian-Guo LI,Dong-Mei BAI,Li-Ying YAN,Li-Yun WAN,Yan-Ping KANG,Dong-Xin HUAI,Yong LEI,Bo-Shou LIAO

表1 克隆AhFAD2基因使用的引物及其序列

Table 1 Sequences of primers for cloning of AhFAD2

基因 Gene 基因注册号 Accession No. 上游引物 Forward primer (5°-3°) 下游引物 Reverse primer (5°-3°) AhFAD2-1A Aradu.G1YNF GATTATTGACTTGCTTTGTAGTAGTGC ACACAAACGTTTTCAACTCTGAC AhFAD2-1B Araip.S3GXY CAGAACCATTAGCTTTGTAGTAGTGC ACACAAACGTTTCCAACTCTGAC AhFAD2-2 Araip.65EGG CAGAACCATTAGCTTTGTAGTAGTGC ACACAAACGTTTCCAACTCTGAC AhFAD2-3A Aradu.10027244 AGCACAACCGATAATACTCTCCCAA TTATTCTGAGCACAACCGATAATA AhFAD2-3B Araip.D6HPL CTCTGTTCCTCCTTCAAACAGGACG TAATCAACCATCCTAATTGGCAGAA AhFAD2-4A Aradu.7W39T ATGGGGTCTGGGGGTCATTCTTCCG TTAAAGCTTATTATACCAAAGAACACCTTT AhFAD2-4B Araip.WI51C ATGGGGTCTGGTGGTCATTCTG TTAAAGCTTATTATACCAAAGAACACCT