花生FAD2基因家族表达分析及其对低温胁迫的响应
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薛晓梦,李建国,白冬梅,晏立英,万丽云,康彦平,淮东欣,雷永,廖伯寿
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Expression profiles of FAD2 genes and their responses to cold stress in peanut
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Xiao-Meng XUE,Jian-Guo LI,Dong-Mei BAI,Li-Ying YAN,Li-Yun WAN,Yan-Ping KANG,Dong-Xin HUAI,Yong LEI,Bo-Shou LIAO
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表2 Real time PCR引物及其碱基序列
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Table 2 Sequences of primers for real-time PCR
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基因
Gene | 引物名称
Primer name | 引物序列
Primer sequence (5°-3°) | | AhFAD2-1A and AhFAD2-1B | RTAhFAD2-1-F | TTTATGTCTCAGATTCATCTGT | | RTAhFAD2-1-R | CCTCTTAACCAGTCCCATTCG | | AhFAD2-2 | RTAhFAD2-2-F | TTCTAATTTATGTCTCAGGACTCAG | | RTAhFAD2-2-R | CCATAATCTCTGTCCACTGTTGCCA | | AhFAD2-3A and AhFAD2-3B | RTAhFAD2-3-F | GGTCTTATCCGTCTTGTCATGG | | RTAhFAD2-3-R | AGATGAATCGTAATGTGGCAATG | | AhFAD2-4A and AhFAD2-4B | RTAhFAD2-4-F | TGGCCTTTGTACTGGTTCG | | RTAhFAD2-4-R | GGACGAGAAGGAAAGAATGGAG | | AhActin | Actin-F | TAAGAACAATGTTGCCATACAGA | | Actin-R | GTTGCCTTGGATTATGAGC |
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