一个新的玉米silky1基因等位突变体的遗传分析与分子鉴定

王晓娟,潘振远,刘敏,刘忠祥,周玉乾,何海军,邱法展

Genetic analysis and molecular characterization of a new allelic mutant of silky1 gene in maize

Xiao-Juan WANG,Zhen-Yuan PAN,Min LIU,Zhong-Xiang LIU,Yu-Qian ZHOU,Hai-Jun HE,Fa-Zhan QIU

表3 连锁标记信息以及与突变位点的交换频率

Table 3 Information and exchange frequency between linkage markers and mutation sites

标记Primer 左引物序列 Left sequence (5°-3°) 右引物序列 Right primer sequence (5°-3°) 物理位置 Physical location 交换频率 Exchange frequency C6-24 ATTCGATCTAGGGTTTGGGTTCAG GATGCAGTAGCATGCTGGATGTAG 68,453,519 0.045 C6-30 GGAGGAGTACGGCTTCGAG AAGTGGTTGCTGTTGTGGTG 87,429,100 0 C6-34 CTGATCGTGGATATCCCCTCC GACTCGAAGCCAAAACCAACTC 98,078,061 0.011 C6-40 CGCTGAGGCTTAAGATGGTGTT AACGCCTTTACGAGCACGAAC 107,626,979 0.034