甘蓝SI相关基因BoCDPK14的克隆与分析
白晓璟,廉小平,王玉奎,张贺翠,刘倩莹,左同鸿,张以忠,谢琴琴,胡燈科,任雪松,曾静,罗绍兰,蒲敏,朱利泉

Cloning and analysis of BoCDPK14 in self-incompatibility Brasscia olerace
Xiao-Jing BAI,Xiao-Ping LIAN,Yu-Kui WANG,He-Cui ZHANG,Qian-Ying LIU,Tong- Hong ZUO,Yi-Zhong ZHANG,Qin-Qin XIE,Deng-Ke HU,Xue-Song REN,Jing ZENG,Shao-Lan LUO,Min PU,Li-Quan ZHU
表1 基因克隆及定量PCR中的引物
Table 1 Primers used in gene cloning and qRT-PCR
引物名称
Primer		 引物序列
Primer sequence (5°-3°)		 用途
Use
GST
 F: GATCTGGTTCCGCGTGGATCCATGGGGAATTGCTGTGGAAC
R: GATGCGGCCGCTCGAGTCGACCTCTGCATCGCGATTATTAGAA		 基因的原核表达
Prokaryotic expression
QRP
 F: TCAAGAAAAGAGCACTCAGGG
R: GATGTGTACAGATATGGCTACGAAC		 荧光定量PCR引物
qRT-PCR
DActin
 F: GGCTGATGGTGAAGATATTCA
R: CAAGCACAATACCAGTAGTAC		 扩增内参基因
For the internal control
CDPK14-GFP
 F: AAGTCCGGAGCTAGCTCTAGAATGGGGAATTGCTGTGGAAC
R: GCCCTTGCTCACCATGGATCCCTCTGCATCGCGATTATTAGAA		 基因亚细胞定位
Subcellular location
CDPK14Δ-BD
 F: AGGACCTGCATATGGCCATGGATGGGGAATTGCTGTGGAAC
R: CCGCTGCAGGTCGACGGATCCTAACCATGGATGTTCAAGCACTT		 酵母表达
Yeast expression
GLR2.8d-AD
 F: GTACCAGATTACGCTCATATGAGCCCGACAAGTGAAATTAAAGTAG
R: ATGCCCACCCGGGTGGAATTCTTTAAGGAACACCCATGTGTTCTTG		 酵母表达
Yeast expression