甘蓝SI相关基因BoCDPK14的克隆与分析

白晓璟,廉小平,王玉奎,张贺翠,刘倩莹,左同鸿,张以忠,谢琴琴,胡燈科,任雪松,曾静,罗绍兰,蒲敏,朱利泉

Cloning and analysis of BoCDPK14 in self-incompatibility Brasscia olerace

Xiao-Jing BAI,Xiao-Ping LIAN,Yu-Kui WANG,He-Cui ZHANG,Qian-Ying LIU,Tong- Hong ZUO,Yi-Zhong ZHANG,Qin-Qin XIE,Deng-Ke HU,Xue-Song REN,Jing ZENG,Shao-Lan LUO,Min PU,Li-Quan ZHU

图10 酵母双杂交验证CDPK14Δ和GLR2.8d之间的相互作用 第1排表示pGADT7-BoGLR2.8d×pGBDT7-BoCDPK14Δ; 第2排表示阳性对照(pGADT7-T×pGBDT7-p53); 第3排表示阴性对照(pGADT7-T×pGBDT7-Lam)。

Fig. 10 Interaction of CDPK14Δ and GLR2.8d in yeast two-hybrid assay The first row indicates pGADT7-BoGLR2.8d×pGBDT7-BoCDPK14Δ; the second row indicates positive control (pGADT7-T×pGBDT7-p53); the third row indicates negative control (pGADT7-T×pGBDT7-Lam). DDO: SD/-Leu/-Trp; TDO: SD/-Leu/-Trp/-His; QDO/X/A: SD/-Leu/-Trp/-His/-Ade/X-α-gal/AbA.