甘蓝SI相关基因BoCDPK14的克隆与分析

白晓璟,廉小平,王玉奎,张贺翠,刘倩莹,左同鸿,张以忠,谢琴琴,胡燈科,任雪松,曾静,罗绍兰,蒲敏,朱利泉

Cloning and analysis of BoCDPK14 in self-incompatibility Brasscia olerace

Xiao-Jing BAI,Xiao-Ping LIAN,Yu-Kui WANG,He-Cui ZHANG,Qian-Ying LIU,Tong- Hong ZUO,Yi-Zhong ZHANG,Qin-Qin XIE,Deng-Ke HU,Xue-Song REN,Jing ZENG,Shao-Lan LUO,Min PU,Li-Quan ZHU

图3 甘蓝BoCDPK14 CDNA结构图及其推导的氨基酸序列 下划直线为酪蛋白激酶II磷酸化位点, 虚线框为蛋白激酶C磷酸化位点, 椭圆为N-糖基化位点, 实线框为cAMP或cGMP依赖性蛋白激酶磷酸化位点, 双线框表示N-肉豆蔻酰化位点, 圆点为EF-hand钙结合结构域, 双线表示蛋白激酶ATP结合区, 星星表示丝氨酸/苏氨酸蛋白激酶活性位点。

Fig. 3 Gene structure of Brassica oleracea BoCDPK14 and its deduced amino acid sequence The underline show protein kinase II phosphorylation site; the dashed box show protein kinase C phosphorylation site; the oval show N-glycosylation site; the box show cAMP or cGMP-dependent protein kinase phosphorylation site; the double box show N-myristoylation site; the dot show the EF-hand calcium binding domain; double line show protein kinase ATP binding domain; stars show serine/threonine protein kinase active site.