大豆抗SC3候选基因的克隆及分析

向文扬,杨永庆,任秋燕,晋彤彤,王丽群,王大刚,智海剑

Cloning and analysis of candidate gene resistant to SC3 in soybean

Wen-Yang XIANG,Yong-Qing YANG,Qiu-Yan REN,Tong-Tong JIN,Li-Qun WANG,Da-Gang WANG,Hai-Jian ZHI

表1 基因克隆引物及荧光定量引物

Table 1 Primers in gene cloning and qPCR

引物名称 Primer name 上游引物 Forward primer (5'-3') 下游引物 Reverse primer (5'-3') GmR47-1 TTTCAGAATCTATTGATATTAGGG TCTGTGAAACTATGCCTTGC GmR51-1 GGATTGGAGTGGGAGGA GGGTTGAAGCAACGAAA GmR47-2 TGGGATTGGAGTGGGAGGAGT CCATCAAAGTTGAATCCCAATTCAC GmR51-2 GGGAGGAGTTGAATTAGCG CCAGTCTCCACCACTTTGTT GmR47/51-qPCR GTAGGGGTCTTTGTTGATGTTGATT TCTGAGAAACTCCTATGTCCGCTA Tubulin-qPCR AGTATGAGGACGAGGAGGACGAT TACGCATCACATAGCATAAGTAAGACAC