大豆抗SC3候选基因的克隆及分析

向文扬,杨永庆,任秋燕,晋彤彤,王丽群,王大刚,智海剑

Cloning and analysis of candidate gene resistant to SC3 in soybean

Wen-Yang XIANG,Yong-Qing YANG,Qiu-Yan REN,Tong-Tong JIN,Li-Qun WANG,Da-Gang WANG,Hai-Jian ZHI

表2 可变剪接检测引物及荧光定量引物

Table 2 Primer used in alternative splicing detection and qPCR

引物名称 Primer name 引物 Primer (5'-3') Intron1-Uni F: TTCGGAGGCGATTGAACAT R: TGCTCAATTTGATCAATTAATTAAACTCA Intron2-Uni F: AAATTGTTCGACAGGAATCACC R: TGGCAGAAACTCGACGTAATG Intron3-Uni F: CTCTTGAATCCACCAAAGCAGG R: AGTTCATATGATTAGAAGAACATGTAGAG Intron1-Spe F: ATCTACTAGTCATATGGATTTGCTCAATTTGATCAATTAATTAAACTCA R: CGGTACCCGGGGATCCGATTTTCGGAGGCGATTGAACAT Intron2-Spe F: CGGTACCCGGGGATCCGATTAAATTGTTCGACAGGAATCACC R: ATCTACTAGTCATATGGATTTGGCAGAAACTCGACGTAATG Intron3-Spe F: CGGTACCCGGGGATCCGATTCTCTTGAATCCACCAAAGCAGG R: ATCTACTAGTCATATGGATTAGTTCATATGATTAGAAGAACATGTAGAG Intron1-qPCR F: GAAATGGAGGAACGCACTGC R: AAGATCGACAAGAAGATATTACCTGAA Intron2-qPCR F: GGCAAACGCAGTAGGTTATGG R: ATACCATCAATAGAATGGCAGAAACT Intron3-qPCR F: ATGTAGGAGAATGCTACTGAAACAGG R: AGAATATCTCTTATTTCCAGCCTCAT