小麦粒重相关基因TaCYP78A5功能标记开发及验证

司文洁,吴林楠,郭利建,周梦蝶,刘香利,马猛,赵惠贤

Development and validation of the functional marker of grain weight-related gene TaCYP78A5 in wheat (Triticum aestivum L.)

Wen-Jie SI,Lin-Nan WU,Li-Jian GUO,Meng-Die ZHOU,Xiang-Li LIU,Meng MA,Hui-Xian ZHAO

表1 用于TaCYP78A5分析的引物

Table 1 Primers used inTaCYP78A5 analysis

引物名称 Primer name 正向引物序列 Forward primer sequence (5′-3′) 反向引物序列 Reverse primer sequence (5′-3′) A5-Ap AGCCCCTTCATCTGTCGGGTAACCC TTAGCCGGGAGGAGGAGCAGGAG A5-Bp GAAAAGGCGAACCACGGATCATG TAGCCGGGGGAGGAGCAGGAG A5-Dp CTTGGCGAAGCCCTGCCGAGATC CGCCCGTGAGAGCACAGGAGT GADPH CCTTCCGTGTTCCCACTGTTG ATGCCCTTGAGGTTTCCCTC A5-A-RT CCATTCCTCAAGTGGCTCGAT TGGGTGCACCACCATCCTC A5-B-RT TCATTCCTCAAGTGGCTCGAC GGGTGCAGCACCATCCTG A5-D-RT TCCTGCTCGCCGTGCTC TTGCGCGGTGGAAGAGG CAPS-A5-Ap TACAAAGCCGGAGCGCCTTCGCGTTCTTC GAAGCTGGCTCCTCCCGGCCTGCG