砷胁迫下甘蓝型油菜苗期根、下胚轴和鲜重的全基因组关联分析

曲存民,马国强,朱美晨,黄小虎,贾乐东,王书贤,赵会彦,徐新福,卢坤,李加纳,王瑞

Genome-wide association of roots, hypocotyls and fresh weight at germination stage under as stress in Brassica napus L.

Cun-Min QU,Guo-Qiang MA,Mei-Chen ZHU,Xiao-Hu HUANG,Le-Dong JIA,Shu-Xian WANG,Hui-Yan ZHAO,Xin-Fu XU,Kun LU,Jia-Na LI,Rui WANG

表1 候选基因qRT-PCR特异性引物序列

Table 1 Primers sequences of candidate genes for qRT-PCR

基因名 Gene name 基因号 Gene ID 引物序列 Sequences of primers (5'-3') BnaABCG25 BnaA02g15690D F: GTGGCACTCAGATTATCGAGACGTACA R: AGTCCGAGTCCTTGGGATGCTAAAA BnaGSTU10 BnaA07g31660D F: ATCGATGAAACCTGGAAGAACT R: GAATCTCTCTCTAGCCACTTCC BnaGST16 BnaA02g20640D F: GCAGGTATCAAAGTTTTCGGACACG R: GTGGGATATGTTCTTGGAGTCGGCT BnaPHT3;3 BnaA06g25890D F: GTGAAGCAACAGAAAGTAACGGTGGT R: CCGCCGCTGTTGTTGTTGTTAC BnaPHT1;9 BnaA07g32730D, BnaA07g32740D, BnaA07g32750D F: CTCCACGGCCGTGACCTCTTC R: AACCCCATTATCTGAATCTTGACTCG BnaGSTU25 BnaA09g45070D, BnaA09g45080D, BnaA09g45090D F: TCCTTCCTTCTGATCCTTACCA R: GGACACTCTGCTTCGATGCTAA BnaGSTU12 BnaC06g31020D, BnaC06g31030D F: TCAGATCCTTCCATCCTCCCCTCA R: CCACCATAGCCCCACAAGCGAT BnaGSTU11 BnaC06g31040D F: CCCCGTCCATAAACAGATCCCC R: CGCTATTGCCATCTTTCTTTCCTCC BnaUBC21 BnaA06g27860D, BnaA09g04490D F: CCTCTGCAGCCTCCTCAAGT R: CATATCTCCCCTGTCTTGAAATGC BnaACTIN7 BnaA03g55890D, BnaC02g00690D, BnaA10g22340D F: CCCTGGAATTGCTGACCGTA R: TGGAAAGTGCTGAGGGATGC