两份玉米CMS-C恢复系的育性恢复力测定及恢复基因的分子标记定位

牟碧涛,赵卓凡,岳灵,李川,张钧,李章波,申汉,曹墨菊

Identification of fertility restoration and molecular mapping of restorer genes in two maize restore lines of CMS-C

Bi-Tao MOU,Zhuo-Fan ZHAO,Ling YUE,Chuan LI,Jun ZHANG,Zhang-Bo LI,Han SHEN,Mo-Ju CAO

附表2 用于7250-14-1恢复基因定位的多态性引物

Supplementary table 2 Polymorphic markers for restoring gene mapping in 7250-14-1

引物 Primer 引物类型 Primer type 正向序列 Forward sequence (5'-3') 反向序列 Reverse sequence (5'-3') m-10 SSR AGCGCTCGATTCCTGTAGTG GGGTGTCGTTGGTTGGGATT Chr8-86080 InDel CGTCGTTGAGGTGAGAAGAG CTCCGAACCTGATCCGAGTA B-2 InDel ACGAATACGATACGTAGCCA GTGAATCTGCGGTGAACAAA B-6-2 InDel CCAATGTTTTGATGGAAGTCCT AATTGCCATGTTCTTACCTGT B-1 InDel GATCGTTCCGGCCCAAGAAG TAGCCGTGGAGTTGGTAGCC Chr8-398180 InDel GCCAGTTCGGAGACAGGAT ACCGCCATCCAATTAACAAG IDP8319 InDel TTGACCCTCCTGTTACGTGC GAGCATGGACCACATGACC IDP500 InDel CACTGCCGTAGAGTAGTGCG GGCTTCAAGATCAGTCCG IDP7866 InDel GGACGAAGCGATCGAGTACC AGATGAGGGAAGTGAGCAGC Chr8-1330080 InDel CCAAGTTGGATACAACGACAGA AGAAGCAACGTCTGCAGGAT