挥发性抑芽物质对马铃薯块茎萌芽的影响及其作用机制
邹雪,丁凡,余金龙,彭洁,邓孟胜,王宇,刘丽芳,余韩开宗,陈年伟,王西瑶

Suppression mechanism of volatile sprout-inhibitors on potato tuber sprouting
Xue ZOU,Fan DING,Jin-Long YU,Jie PENG,Meng-Sheng DENG,Yu WANG,Li-Fang LIU,Kai-Zong YU-HAN,Nian-Wei CHEN,Xi-Yao WANG
表1 试验选择基因的引物序列
Table 1 Primer sequences used in this study
基因
Gene
编号
ID
引物
Primers (5°-3°)
长度
Length
(bp)
熔解温度
Melt temp.
(℃)
延伸因子EF1αL (内参Reference gene)
Elongation factor 1 alpha-like
PGSC0003DMT400014674 CTTGTACACCACGCTAAGGAG
GTCAATGCAAACCATTCCTTG
155 81.5
果胶裂解酶 PEL
Pectate lyase
PGSC0003DMT400051938 TGGGAAGGATCATGGAAACA
ACCAGCATTTGAGGTAATCATT
168 83.0
果胶酯酶PME
Pectinesterase
PGSC0003DMT400005343 AATCTACTGGTGTCGCTTAC
TATTGTGGCATTGGTTCA
167 78.5
多聚半乳糖醛酸酶PG
Polygalacturonase
PGSC0003DMT400035162 CAAGTAAAGGCTACGACAA
TATGGACCTGAGAATGAAA
199 81.0
细胞色素P450, CYP77A6
Cytochrome P450, CYP77A6
PGSC0003DMT400060106 CTTAGTCCACGAAGCATT
TTCCGTAGTGACCTCCA
141 84.0
ω-羟基酸O-阿魏酸酯转移酶HPFT
Omega-hydroxypalmitate O-feruloyl
transferase
PGSC0003DMT400020180 AGGTCACTTATCCGTTCC
GAGTTGGCAATTTCAGG
110 79.0
甘油二酯O-酰基转移酶WES
Diacylglycerol O-acyltransferase
PGSC0003DMT400023554 GTGTTGACCCGATTACCCA
CTTGTTTAGTGACAGCCTCTT
295 81.5
AG-基序结合蛋白 GATA4L
GATA transcription factor 4-like
PGSC0003DMT400030708 GGTTGTTACTACGATGCTCT
AATTATTGGATGTATCTTCCAC
121 80.5
Kip相关蛋白4 KRP4
Kip-related protein 4
PGSC0003DMT400019919 AAAAGACAGAATCGGAGTT
GTCCTCTCTCTACCATCAAT
182 81.0
氨基环丙烷羧酸氧化酶ACO
Aminocyclopropanecarboxylate oxidase
PGSC0003DMT400030676 CAGAGGAGCATCGTTACTGG
AAAGAACCCTGCCTCCAAAC
194 82.0
转录因子WRKY 75
WRKY transcription factor 75
PGSC0003DMT400056352 CAGCATCATCGTCGTCAT
CATTAGTCCCAAGAACCC
131 77.5
病程相关蛋白STH-2
Pathogenesis-related protein STH-2
PGSC0003DMT400003934 AAAACCAGGCATGGAACT
ACGTGTAGACCTGATTCTTT
99 79.0
呼吸爆发氧化酶(NADPH氧化酶) RBOH
Respiratory burst oxidase (NADPH oxidase)
PGSC0003DMT400066027 CTCTCATCACCATGCTCCAGG
TGGCAGTGTCGTCTACCATAT
168 82.5