青海高原耐旱蚕豆品种青海13号响应干旱胁迫蛋白质组学分析

李萍,侯万伟,刘玉皎

Proteomic analysis of drought stress response on drought resistance for Vicia faba L. variety ‘Qinghai 13’ in Qinghai Plateau of China

Ping LI,Wan-Wei HOU,Yu-Jiao LIU

表1 干旱胁迫下蚕豆叶片中差异表达蛋白的MALDI-TOF/TOF鉴定结果

Table 1 Identification of differential proteins by MALDI-TOF/TOF in broad bean leaf under drought stress

蛋白点 Spot No. 登录号 Accession No. 分子量/等电点 Theoretical Mr (kD)/pI 序列覆盖率 Sequence coverage (%) 评分 Score 蛋白名称 Protein name 物种来源 Source of species A, up-regulated protein spots 2705 gi|15667623 15.923/5.78 18 230 Drought inducible 22 kD protein 甘蔗Saccharum officinarum 3106 gi|380005612 20.731/5.79 31 717 Superoxide dismutase 蚕豆Vicia faba 3208 gi|83776798 16.256/5.82 19 332 17.5 kDa class I HSP, partial 花生Arachis hypogaea 3809 gi|308810206 85.783/8.69 1 54 Shikimate dehydrogenase substrate binding,N-terminal, partial (ISS) 绿藻类Ostreococcus tauri 5605 gi|357508933 39.616/5.91 9 158 O-acetylserine (thiol) lyase 蒺藜状苜蓿Medicago truncatula 5807 gi|357481949 65.668/5.7 5 237 Stress-inducible protein, putative 蒺藜状苜蓿Medicago truncatula 7206 gi|75220301 23.908/6.16 13 212 Full=Kunitz-type trypsin inhibitor-like 2 protein; 豌豆Pisum sativum 408 gi|571556750 21.914/4.36 35 493 Nascent polypeptide-associated complex subunit alpha-like protein 1 大豆Glycine max B, down-regulated protein spots 1815 gi|3913031 56.446/5.34 13 387 Full=1,4-alpha-D-glucan maltohydrolase 紫花苜蓿Medicago sativa 2306 gi|115788 28.692/5.47 25 411 Full=Chlorophyll a-b binding protein AB80, chloroplastic; 豌豆Pisum sativum 2309 gi|115788 28.692/5.47 25 415 Full=Chlorophyll a-b binding protein AB80, chloroplastic; 豌豆Pisum sativum 2813 gi|1045394920 86.972/5.46 13 643 Hypothetical protein TSUD_183880 三叶草Trifolium subterraneum 3815 gi|528749836 55.812/5.22 20 659 ATP synthase CF1 alpha subunit (plastid) 蚕豆Vicia faba 3816 gi|528749836 55.812/5.22 20 760 ATP synthase CF1 alpha subunit (plastid) 蚕豆Vicia faba 4707 gi|75308025 43.565/5.5 17 323 Full=S-adenosylmethionine synthase 2; Short=AdoMet synthase 2; 茱萸Elaeagnus umbellata 5202 gi|217071344 31.249/6.59 8 309 Unknown 蒺藜状苜蓿Medicago truncatula 5314 gi|729390274 29.386/8.65 16 426 Chlorophyll a-b binding protein 8, chloroplastic-like 醉蝶花Tarenaya hassleriana 5808 gi|357445031 80.087/6 11 480 Plastid transketolase 蒺藜状苜蓿Medicago truncatula 5809 gi|357445031 80.087/5.78 11 532 Plastid transketolase 蒺藜状苜蓿Medicago truncatula 6303 gi|502090577 37.493/7.04 25 570 Carbonic anhydrase, chloroplastic isoform X1 鹰嘴豆Cicer arietinum 7005 gi|132097 20.402/9.24 39 354 Full=Ribulose bisphosphate carboxylase small chain 3C, chloroplastic; Short=RuBisCO small subunit 3C; AltName: Full=PSS15; Flags: Precursor 豌豆Pisum sativum 7307 gi|502090577 37.493/7.04 31 723 Carbonic anhydrase, chloroplastic isoform X1 鹰嘴豆Cicer arietinum 7311 gi|502090577 37.493/7.04 31 735 Carbonic anhydrase, chloroplastic isoform X1 鹰嘴豆Cicer arietinum 7608 gi|20729 43.696/8.93 24 847 Unnamed protein product 豌豆Pisum sativum 8004 gi|132097 20.402/9.24 31 448 Full=Ribulose bisphosphate carboxylase small chain 3C, chloroplastic; Short=RuBisCO small subunit 3C; AltName: Full=PSS15; Flags: Precursor 豌豆Pisum sativum 8010 gi|502082899 19.055/9.93 20 219 Photosystem II repair protein PSB27-H1, chloroplastic 鹰嘴豆Cicer arietinum 1404 gi|502133626 37.498/7.63 29 541 Haloacid dehalogenase-like hydrolase domain-containing protein At3g48420 鹰嘴豆Cicer arietinum