通过分子标记辅助选择将耐储藏主效QTL qSS-9 Kas转入宁粳4号提高其种子贮藏能力

张平,姜一梅,曹鹏辉,张福鳞,伍洪铭,蔡梦颖,刘世家,田云录,江玲,万建民

Introducing qSS-9 Kas into Ningjing 4 by molecular marker-assisted selection to improve its seed storage ability

Ping ZHANG,Yi-Mei JIANG,Peng-Hui CAO,Fu-Lin ZHANG,Hong-Ming WU,Meng-Ying CAI,Shi-Jia LIU,Yun-Lu TIAN,Ling JIANG,Jian-Min WAN

图3 各亲本和BC5F2在与qSS-9紧密连锁的分子标记Y-10、Y-11、Y-14、Y-13下的基因型鉴定 A: 利用与qSS-9紧密连锁的分子标记Y-10、Y-11、Y-14和Y-13对各亲本的基因型进行鉴定; 其中, 1: DNA marker; 泳道2~5、6~9、10~13和14~17分别代表各品系在Y-10、Y-11、Y-14和Y-13的基因型; 泳道2、6、10和14为Nipponbare; 泳道3、7、11和15为宁粳4号; 泳道4、8、12和16为置换系SL36; 泳道5、9、13和17为Kasalath。B: 利用与qSS-9紧密连锁的分子标记Y-10、Y-11、Y-14和Y-13对宁粳4号、SL36及BC2F1间的基因型进行鉴定: 其中1: DNA marker; 泳道2~6分别代表宁粳4号、SL36及BC2F1在Y-10下的基因型; 7~11分别代表宁粳4号、SL36及BC5F2在Y-11下的基因型; 12~16分别代表宁粳4号、SL36及BC2F1在Y-14下的基因型; 17~21分别代表宁粳4号、SL36及BC2F1在Y-13下的基因型。

Fig. 3 Genotype identification of parents and BC5F2 by molecular markers Y-10, Y-11, Y-14, and Y-13 close linked to qSS-9 A: Genotype identification of parents by molecular markers Y11 and Y14 close linkage to qSS-9; 1: DNA marker; 2-5, 6-9, 10-13, and 14-17 indicate genotype of markers Y-10, Y-11, Y-14, and Y-13 respectively; 2, 6, 10, and 14 indicate genotype of Nipponbare; 3, 7, 11, and 15 indicate genotype of Ningjing 4; 4, 8, 12, and 16 indicate genotype of SL36; 5, 9, 13, and 17 indicate genotype of Kasalath, respectively. B: Genotype identification of Ningjing 4, SL36 and BC5F2 by molecular markers Y11 and Y14 close linked to qSS-9; 1: DNA marker; 2-6 : Genotype of Ningjing 4, SL36 and BC5F2 under marker Y-10; 7-11: Genotype of Ningjing 4, SL36 and BC5F2 under marker Y-11; 12-16: Genotype of Ningjing 4, SL36 and BC5F2 under marker Y-14; 17-21: Genotype of Ningjing 4, SL36 and BC5F2 under marker Y-13, respectively.