通过分子标记辅助选择将耐储藏主效QTL qSS-9 Kas转入宁粳4号提高其种子贮藏能力

张平,姜一梅,曹鹏辉,张福鳞,伍洪铭,蔡梦颖,刘世家,田云录,江玲,万建民

Introducing qSS-9 Kas into Ningjing 4 by molecular marker-assisted selection to improve its seed storage ability

Ping ZHANG,Yi-Mei JIANG,Peng-Hui CAO,Fu-Lin ZHANG,Hong-Ming WU,Meng-Ying CAI,Shi-Jia LIU,Yun-Lu TIAN,Ling JIANG,Jian-Min WAN

图4 亲本和后代种子在不同老化处理下的发芽率和丙二醛的含量 A: 宁粳4号、SL36、BC3F2 (Y6647)在自然老化一年MDA含量; B: 宁粳4号、SL36、BC3F2在自然老化一年和两年的发芽率; C: 亲本和BC5F1种子在人工老化处理35 d后的发芽率; D: 宁粳4号、Y3059-1-7、Y3059-1-8、Y3059-1-9的发芽率; E~H: 老化处理30 d后的发芽情况。E、F、G、H分别代表宁粳4号、Y3059-1-7、Y3059-1-8、Y3059-1-9。*表示在P = 0.05水平上差异显著; **表示在P = 0.01水平上差异极显著。

Fig. 4 Germination rate and MDA contents in seeds of Ningjing 4, SL36, and progenies of different generations A: Contents of MDA in seeds of Ningjing 4, SL36, and BC3F2 after natural aging for one year; B: Germination rate of Ningjing 4, SL36 and BC3F2 after natural aging forone year and two years; C: Germination rates of parents and BC5F1 after artificial aging for 35 days; D: Seed germination of BC5F2 and Ningjing 4 after accelerated aging for 30 d and storage at 40°C and 80% RH; E-H: Seed viability of the control after aging treatment; E, F, G, and H represent Ningjing 4, Y3059-1-7, Y3059-1-8, Y3059-1-9, respectively. Significant differences relative to the control were assessed by t-tests. * and **, significant at P = 0.05 and P = 0.01, respectively. Values are means ± SD (n = 2).