通过分子标记辅助选择将耐储藏主效QTL qSS-9 Kas转入宁粳4号提高其种子贮藏能力

张平,姜一梅,曹鹏辉,张福鳞,伍洪铭,蔡梦颖,刘世家,田云录,江玲,万建民

Introducing qSS-9 Kas into Ningjing 4 by molecular marker-assisted selection to improve its seed storage ability

Ping ZHANG,Yi-Mei JIANG,Peng-Hui CAO,Fu-Lin ZHANG,Hong-Ming WU,Meng-Ying CAI,Shi-Jia LIU,Yun-Lu TIAN,Ling JIANG,Jian-Min WAN

图5 宁粳4号和Y3059的TTC试验、直链淀粉含量和株型的比较 A: 老化处理30 d后宁粳4号和Y3059的TTC染色情况, 从左到右分别代表宁粳4号、Y3059-1-7、Y3059-1-8、Y3059-1-9; B: Y3059及宁粳4号种子中TTCH的含量; C: 宁粳4号和Y3059老化处理前后表观淀粉含量的变化; D: 抽穗时期宁粳4号(左)和BC5F2 (右)株型; E: 抽穗35 d后宁粳4号(左)和BC5F2 (右)株型。*表示在P = 0.05水平上差异显著; **表示在P = 0.01水平上差异极显著。

Fig. 5 Comparison of Ningjing 4 and Y3059 in tetrazolium assays, apparent amylose content and plant types A: Tetrazolium assay of Ningjing 4 and Y3059; From left to right represent Ningjing 4, Y3059-1-7, Y3059-1-8, Y3059-1-9, respectively; B: The content of TTCH of Y3059 and Ningjing 4; C: The change of apparent amylose content in Ningjing 4 and Y3059; D: Plant type of Ningjing 4 and BC5F2 at heading; E: Plant type of Ningjing 4 and BC5F2 at 35 days after heading; Significant differences relative to the control were assessed by t-tests. * and **, significant at P = 0.05 and P = 0.01, respectively. Values are means ± SD (n = 2).