甘蓝型油菜NRT1.5和NRT1.8家族基因的生物信息学分析及其对氮-镉胁迫的响应

梁桂红,华营鹏,周婷,廖琼,宋海星,张振华

Bioinformatics analysis and response to nitrate-cadmium stress of NRT1.5 and NRT1.8 family genes in Brassica napus

Gui-Hong LIANG,Ying-Peng HUA,Ting ZHOU,Qiong LIAO,Hai-Xing SONG,Zhen-Hua ZHANG

图9 甘蓝型油菜NRT1.5和NRT1.8基因对镉胁迫的响应及基因共表达网络分析 图a和图b分别表示甘蓝型油菜地上部和根部NRT1.5家族基因在镉处理时的基因表达丰度; 图c表示NRT1.5家族基因在镉处理时的基因共表达网络分析。图d和图e分别表示甘蓝型油菜地上部和根部NRT1.8家族基因在镉处理时的基因表达丰度; 图f表示NRT1.8家族基因在镉处理时的基因共表达网络分析。FPKM表示每千个碱基转录每百万映射读取的fragments。图中所示显著性差异是单个基因对照和镉处理两两对比, 未达到显著性差异的图中未标记。误差线代表3个独立生物学重复的标准误, a和b表示差异显著(Ρ < 0.05)。

Fig. 9 Response to cadmium stress and co-expression network analysis of NRT1.5 and NRT1.8 genes Fig. a and Fig. b show the gene expression abundance of NRT1.5 family genes in shoots and roots of B. napus with cadmium stress. Fig. c shows the co-expression network analysis of NRT1.5 family genes with cadmium stress. Fig. d and Fig. e show the gene expression abundance of NRT1.8 family genes in shoots and roots of B. napus with cadmium stress. Fig. f shows the co-expression network analysis of NRT1.8 family genes with cadmium stress. FPKM means the fragments per kilobase of exon model per million mapped reads. The significant difference shown in the figure is the pairwise comparison between control and cadmium treatment, and the figure without significant difference is not marked. Data are shown as means±SE (n = 3) and the different lowercases above text are used to show statistical significance (Ρ < 0.05).