棉花中GhTFL1a和GhTFL1c基因的表达及启动子分析

张晓红,胡根海,王寒涛,王聪聪,魏恒玲,付远志,喻树迅

Expression and promoter activity of GhTFL1a and GhTFL1c in Upland cotton

Xiao-Hong ZHANG,Gen-Hai HU,Han-Tao WANG,Cong-Cong WANG,Heng-Ling WEI,Yuan-Zhi FU,Shu-Xun YU

表1 本研究所用引物

Table 1 Primers used in this study

引物名称 Primer name 引物序列 Primer sequence (5′-3′) 用途 Purpose GhTFL1a-up TCCCTGAGCCACTTACCGTTG 荧光定量PCR qRT-PCR GhTFL1a-down AAGCGTCTCTCATGTCGTTG 荧光定量PCR qRT-PCR GhTFL1c-up TCATCTGTTGCCACCAAACCT 荧光定量PCR qRT-PCR GhTFL1c-down TTCCCTTCCAAACGTGGCATC 荧光定量PCR qRT-PCR GhACTIN-up ATCCTCCGTCTTGACCTTG 荧光定量PCR qRT-PCR GhACTIN-down TGTCCGTCAGGCAACTCAT 荧光定量PCR qRT-PCR GhTFL1a-up ATGTCAAGGGTCCCTGAG 基因克隆 Gene cloning GhTFL1a-down TTATCTTCTTCTTGCAGCAGTTTC 基因克隆 Gene cloning GhTFL1c-up ATGGGAGAGCCTCTCATTGTT 基因克隆 Gene cloning GhTFL1c-down TTAGCGTCTCCTTGCAGCAGT 基因克隆 Gene cloning pGhTFL1a-up AAGGAATATAGAGCACAACA 启动子克隆 Promoter cloning pGhTFL1a-down GATGAACAAGACGATGTGTAT 启动子克隆 Promoter cloning pGhTFL1c-up AGTTTAGATTCTTGTGCGAT 启动子克隆 Promoter cloning pGhTFL1c-down TCTTGATGACAGTGAATGAA 启动子克隆 Promoter cloning