水稻短根白化突变体sra1生理生化分析及基因定位

张莉莎,米胜南,王玲,委刚,郑尧杰,周恺,尚丽娜,朱美丹,王楠

Physiological and biochemical analysis and gene mapping of a novel short radicle and albino mutant sra1 in rice

Li-Sha ZHANG,Sheng-Nan MI,Ling WANG,Gang WEI,Yao-Jie ZHENG,Kai ZHOU,Li-Na SHANG,Mei-Dan ZHU,Nan WANG

附表4 用于定量的引物序列

Supplementary table 4 Sequences of primers for RT-PCR

基因 Gene 正向引物 Forward sequence (5′-3′) 反向引物 Reverse sequence (5′-3′) Actin GACCCAGATCATGTTTGAGACCT CAGTGTGGCTGACACCATCAC OsPOLP ACCGGTGCTTTCAGGCTTG GCTGACTGATAATCACACG FtsZ AAAGGACATAACCTTGCAAG AGTTTTCCTATTGAACCGTG OsRPOTP TCCTCATGTCGAGCAAGGAT GAAAGAATGTCTGGACTTTG RpoB TATGGTCTAATTCCGAGCGGT TATGGTCTAATTCCGAGCGG V2 GAGGAGTTCCTCACGATGAT AGCATCAATGATAGACTCC V3 GTTAGATGCTTCACTACACAG GTACCATTGCCAACATGGCAAC HEMA1 CGCTATTTCTGATGCTATGGGT TCTTGGGTGATGATTGTTTGG HEMD AGGGATGGAAGGCTGCTGGA CAGTGGTCCTTGGAAGCTCTG CHLD GCTTGCAGAAAGCTACACAAGC AGGCCGTGAGCTAAAGGAGA CHLM CCATCCATTGGTCTCCTTATGACA GTAGCCTACTTACCATCAATGAGTC CAO1 GACACCTTCATCTGGGCTTCAA CGAGAGACATCCGGTAGAGC PSY1 GTGACCGAGCTCAGCCAG TTAATCGGAAGGCAGATGCT PSY2 GGGCGTTGCGCATCTAGA CAAGAAATCGTGCGAAATCA carb1R AGATGGGTTTAGTGCGACGAG TTTGGGATCGAGGGAGTATTT carb2R TGTTCTCCATGTTCGGCTTCT GCTACGGTCCCCACTTCACT petA TGAATGTGGGTGCTGTTCTTATTT TCGGGCGGCGCTAAT petB TCCTCGGTTCAATACATAATGACCG CGTGCAGGATCATCATTAGAACCA psaA GCGAGCAAATAAAACACCTTTC GTACCAGCTTAACGTGGGGAG psbA CCCTCATTAGCAGATTCGTTTT ATGATTGTATTCCAGGCAGAGC RbcL CTTGGCAGCATTCCGAGTAA ACAACGGGCTCGATGTGATA RbcS TCCGCTGAGTTTTGGCTATTT GGACTTGAGCCCTGGAAGG