玉米穗发芽突变体vp-like8的遗传分析及突变基因鉴定

王瑞,陈阳松,孙明昊,张秀艳,杜依聪,郑军

Genetic analysis and causal gene identification of maize viviparous mutant vp-like8

Rui WANG,Yang-Song CHEN,Ming-Hao SUN,Xiu-Yan ZHANG,Yi-Cong DU,Jun ZHENG

表1 本研究所用的引物

Table 1 Primers used in this study

引物 Primer 正向序列 Forward sequence (5°-3°) 反向序列 Reverse sequence (5°-3°) VP1-G-F1/R1 GCGAGACCTGAAAACACACA CATGGCGTTCTCTAGCATCA VP1-G-F2/R2 CGCACTCCCAAGAGAACC ATAGGGTAAGAGCCCGTGGA VP1-G-F3/R3 GTGGTCGTGAACAGCCAAC GCTCTGCTTCAGCACCTTCT VP1-G-F4/R4 CATCGCTGTCGAGCAATAGA CTGTACCGCATGTTCCACAC VP1-G-F5/R5 TAAAATCGGCCATGGATAGG TCTCTGGCCCAGTGGTTAGT VP1-G-F6/R6 GCTGCTGTTTTCCTCGAATC GCACCTAGCTGCCAAACACT VP1-G-F7/R7 AGTCCTCCGGATCTCTCGTT AAACGGTTGCGTAGATTTGG VP1-G-F8/R8 CCAGTGCAATGTCAGTGCTT AATGGCCGAGAGATCAGGTA VP1-CDS-F1/R1 AGAAGGTGCTGAAGCAGAGC AACGAACAAATTCCCCTGTG Zm-GAPDH-F/R CCCTTCATCACCACGGACTAC AACCTTCTTGGCACCACCCT