水稻温敏型叶片白化转绿突变体tsa2的表型鉴定与基因定位

尚丽娜,陈新龙,米胜南,委刚,王玲,张雅怡,雷霆,林永鑫,黄兰杰,朱美丹,王楠

Phenotypic identification and gene mapping of temperature-sensitive green- revertible albino mutant tsa2 in rice (Oryza sativa L.)

Li-Na SHANG,Xin-Long CHEN,Sheng-Nan MI,Gang WEI,Ling WANG,Ya-Yi ZHANG,Ting LEI,Yong-Xin LIN,Lan-Jie HUANG,Mei-Dan ZHU,Nan WANG

表1 光合色素代谢以及叶绿体发育和光合相关基因的定量引物

Table 1 Primers of photosynthetic pigment metabolism, chloroplast development and photosynthesis-related genes used for quantitative real-time PCR

引物 Primer 正向序列 Forward sequence (5'-3') 反向序列 Reverse sequence (5'-3') UBIQUITIN 5 ACCACTTCGACCGCCACTACT ACGCCTAAGCCTGCTGGTT OsCAO1 GACACCTTCATCTGGGCTTCAA CGAGAGACATCCGGTAGAGC OsCHLI GTTCGAGCCTGGTTTGCTTGC CTCTCCACGGTGTTCCATCCTG OsCHLa/b GGTGGTCAAGGTGTCATTGTCAT ACGCGACTGGATCTTTGGAGAG OsCHLM CCATCCATTGGTCTCCTTATGACA GTAGCCTACTTACCATCAATGAGTC OsCHLD GCTTGCAGAAAGCTACACAAGC AGGCCGTGAGCTAAAGGAGA OsDVR CAGGTCGAGACCGTCAAGAAC ATGACCTGGATCGGCACCTTG OsHEMD TGGAAGGCTGCTGGAAATCCTAAG TCCTTGGAAGCTCTGAGGCCAA OsPSY1 GCCTCAAGCAGGCCTATCATC GTGATGTGCGAGGCATTTGGTC OsPSY2 GACAAATTCTGCGTGCCAGGTT GACAGCAGCTTCTTTGCCTTGTT OsPSY3 TTCAGACAGGCCGAAGAAGGC GTAGGCCCTCTTGGTGAAGTTGT OsPSAA TGGGGTTGATCCTAAGGAGATACCA CCTCCGCGAAAACTAAGAAATTCTG OsPSAB TGGCAGGGCAACGTTTCACAATT CAGTCGCCCAAACAAGATGTCCAAAT OsPSAC GTGTACGAGCTTGTCCAACAGATGTAT GCAGGCGGATTCGCATCTCTTAC OsPSAD AGCAGGTGTTCGAGATGCCCAC ATTGGGGAAGACGCGGTAGAACT OsPSAE AGGCACCAAGGTGAAGATCCTGAG AGGGCGTAGTTGTTGGTCGACAC OsPSAF TCAAGAAGCTCCAGTCGTCGCTC AGGCCGAACTTGCCGTAGTTCTC OsPSAH GTCGCCGTACAACCCTCTCCAG CTTCTTGATCGGAAGCAGGTCCG OsPSAL TCTGAGAAGCCAACGTACCAGGTG AGGTTGGAGAGGTACCAGGCGAC OsPSBA GCGGTTCCCTATTCAGTGCTATG TAACCATGAGCGGCCACAATATT OsPSBB TAGTTTCTGGTTGGGCTGGCTC CTCCAACCACCCCACGAATTG OsPSBD GTAGGATGGTCTGGCCTATTGCTT ACTATTGGCAGGGGTGGAAACTG OsPSBE TGTCTGGAAGCACGGGAGAACGT GTTTGGCCGAGGACTTCCAAACAC OsPSBF CTATTTTTACAGTGCGATGGCTGG TATCGTTGGATGAACTGCATTGCT OsPSBO TCGAGGAGAAGGACGGAATCGAC CTTTGGGTCGAGGAAGGACGAAC OsPSBY TGCGGTCAGACTGAGAGCTTGAG AAGGGAAGAGAGGATTAGGAACAGGATT OsPETA GCAGCAAGGTTATGAAAACCCAC AACAGCACCCACATTCAACCCT OsPETB TTCAGACCTCGCAACCAGACTG AACAAAAGGCAAGGGTTCTTCGA OsPETC CTCAAGGGTGACCCGACGTACCT AGGGGCAGATGAACTTGTTCTCG OsPETD GCGTGGCCCAACGATCTTTTAT TCCAGAGGAGTTGCAAACGGAT OsPETH GTTCTGGGATCCAGGGTAGCAAC ACTGACATGCAGAGCACCTTGTTC OsATPA TGAATCTCCTGCTCCGGGTATAAT TGCTGTTTTGCCGGTTTGTCT OsATPB TCGCAATTCTTGGGTTGGATGA CAACATACTTTCCCGGAGAACCG OsATPG AGGTGGAGCTCCTCTACTCCAAGT TCAGCTTCCCTTCCTTGGTGGT OsNDHA CGAGCTGCCGCTCAATCTATTAG AGGCTGACGCCAAAGATTCCATC OsNDHB TAACAGCTACTCTAGGGGGAATGTT CTTGCCCCACCCATGAGTAAAT OsRBCS2 ACTCCAAGAGCTCGCAGACC GCCTGTAGTTGGCACCCAGA OsLHCA2 CCTGGTGGTCTGTGGTTTGACC CCTGGAACCAAGCTCCCATGACG OsLHCA3 GCTCAGGCTCTTCTCTCTGGGAG CTCAGGGACTGCTTGGATGCGA OsLHCA4 CCCTTCTCCCACCTACCTCAACG TCAACCCGATCTTCGTCAGCACC OsLHCA5 TCACCTCGACGGCACATTACCT CTGCTTCAAACCACACTGGCAG OsLHCB1.3 AAGTGAAGTGGGGGACCGTAGC TCTCGTCGCACTAAACCCATCTTC OsLHCB2.1 CACGATCGAGATGGTGCCAAC CGGCCTGCGGCTTACATTAA OsLHCB3 GACTTCAAGGAGCCCGTGTGG CCATGAGGACGACCTGGAATCC OsLHCB4.1 TCTTTCGCGCGCAATTCAAAC TGCAAGTCGCCATTAACCACC OsLHCB5 GATGAGCTCGCCAAGTGGTACG CTGAGGCCAAAAGGGTCGTAGC