基于Cre/loxP系统的无筛选标记转耐低磷转录因子GmPTF1大豆种质创制与分析

张小芳,董秋平,乔潇,乔亚科,王冰冰,张锴,李桂兰

Creation and analysis of marker free transgenic soybean germplasm with low phosphate tolerance transcription factor GmPTF1 based on Cre/loxP system

Xiao-Fang ZHANG,Qiu-Ping DONG,Xiao QIAO,Ya-Ke QIAO,Bing-Bing WANG,Kai ZHANG,Gui-Lan LI

表1 不同磷浓度下不同大豆植株的根系性状

Table 1 Root character of different soybean seedlings under different phosphorus concentrations

材料 Material 根总长 Root length (cm) 根表面积 Root surface area (cm2) 根平均直径 Root average diameter (mm) 根体积 Root volume (cm3) 正常磷 Normal-P 低磷 Low-P 正常磷 Normal-P 低磷 Low-P 正常磷 Normal-P 低磷 Low-P 正常磷 Normal-P 低磷 Low-P Transgenic line 2515±99 aA 2175±49 aA 146±4.8 aA 113±4.1 aA 0.58±0.04 aA 0.52±0.01 aA 6.67±0.71 aA 4.48±0.29 aA WT 2269±14 bA 1632±29 bB 147±6.5 aA 85±2.2 bB 0.56±0.06 aA 0.48±0.01 bB 6.25±0.95 aA 3.51±0.15 bB