基于Cre/loxP系统的无筛选标记转耐低磷转录因子GmPTF1大豆种质创制与分析

张小芳,董秋平,乔潇,乔亚科,王冰冰,张锴,李桂兰

Creation and analysis of marker free transgenic soybean germplasm with low phosphate tolerance transcription factor GmPTF1 based on Cre/loxP system

Xiao-Fang ZHANG,Qiu-Ping DONG,Xiao QIAO,Ya-Ke QIAO,Bing-Bing WANG,Kai ZHANG,Gui-Lan LI

表2 低磷胁迫对不同大豆幼苗地上部的影响

Table 2 Effects of low phosphorus stress on over ground parts of different soybean seedlings

材料 Material 株高 Plant height (cm) 第一展开叶片面积 The first developed blade area (cm2) 正常磷 Normal-P 低磷 Low-P 降幅 Drop (%) 正常磷 Normal-P 低磷 Normal-P 降幅 Drop (%) Transgenic line 30.3±0.6 aA 24.0±1.2 Aa 20.0 20.28±0.67 aA 19.69±1.51 aA 2.96 WT 37.7±1.5 aA 23.8±2.3 aA 36.9 21.61±1.86 aA 16.06±1.25 bA 25.7