基于Cre/loxP系统的无筛选标记转耐低磷转录因子GmPTF1大豆种质创制与分析

张小芳,董秋平,乔潇,乔亚科,王冰冰,张锴,李桂兰

Creation and analysis of marker free transgenic soybean germplasm with low phosphate tolerance transcription factor GmPTF1 based on Cre/loxP system

Xiao-Fang ZHANG,Qiu-Ping DONG,Xiao QIAO,Ya-Ke QIAO,Bing-Bing WANG,Kai ZHANG,Gui-Lan LI

表3 低磷对不同转基因大豆干物质积累的影响

Table 3 Effects of low P concentrations on dry matter accumulation in shoots and roots of transgenic soybean plants

材料 Material 根干重 Dry weights of roots 茎叶干重 Dry weights of stems and leaves 正常磷 Normal-P 低磷 Low-P 降幅 Drop (%) 正常磷 Normal-P 低磷 Normal-P 降幅 Drop (%) Transgenic line 0.88±0.13 aA 0.61±0.04 aA 30.7 2.81±0.1 aA 1.38±0.06 aA 51 WT 0.85±0.04 aA 0.38±0.06 bB 55.3 2.6±0.25 aA 0.85±0.05 bB 68