基于Cre/loxP系统的无筛选标记转耐低磷转录因子GmPTF1大豆种质创制与分析

张小芳,董秋平,乔潇,乔亚科,王冰冰,张锴,李桂兰

Creation and analysis of marker free transgenic soybean germplasm with low phosphate tolerance transcription factor GmPTF1 based on Cre/loxP system

Xiao-Fang ZHANG,Qiu-Ping DONG,Xiao QIAO,Ya-Ke QIAO,Bing-Bing WANG,Kai ZHANG,Gui-Lan LI

图2 转基因植株删除标记之前的PCR检测图 1: 100 bp DNA ladder marker; 2: 水对照; 3: 野生型对照; 4, 5: P1/P2 PCR产物; 6, 7: P3/P4 nptII PCR产物; 8, 9: P5/P6 GmPTF1基因PCR产物。

Fig. 2 PCR detection of transgenic plants prior to deletion of markers 1: 100 bp DNA marker ladder; 2: blank control; 3: wild type control; 4, 5: PCR products of P1/P2; 6, 7: nptII PCR products of P3/P4; 8, 9: GmPTF1 gene PCR products of P5/P6.