基于Cre/loxP系统的无筛选标记转耐低磷转录因子GmPTF1大豆种质创制与分析

张小芳,董秋平,乔潇,乔亚科,王冰冰,张锴,李桂兰

Creation and analysis of marker free transgenic soybean germplasm with low phosphate tolerance transcription factor GmPTF1 based on Cre/loxP system

Xiao-Fang ZHANG,Qiu-Ping DONG,Xiao QIAO,Ya-Ke QIAO,Bing-Bing WANG,Kai ZHANG,Gui-Lan LI

图4 标记基因删除后重组序列P1/P6 PCR产物测序结果分析 阴影部分为P1和P6引物序列, 线框内为重组拼接位点。

Fig. 4 Analysis of recombinant sequence PCR amplification product of P1/P6 sequencing results after deletion of marker genes The shaded parts were sequences of P1 and P6 primer, and the recombination splice sites were in the frame.