基于Cre/loxP系统的无筛选标记转耐低磷转录因子GmPTF1大豆种质创制与分析

张小芳,董秋平,乔潇,乔亚科,王冰冰,张锴,李桂兰

Creation and analysis of marker free transgenic soybean germplasm with low phosphate tolerance transcription factor GmPTF1 based on Cre/loxP system

Xiao-Fang ZHANG,Qiu-Ping DONG,Xiao QIAO,Ya-Ke QIAO,Bing-Bing WANG,Kai ZHANG,Gui-Lan LI

图5 无筛选标记转GmPTF1基因大豆T5代Southern blot检测图 1: 转基因材料Xba I酶切Southern blot; 2: 转基因材料Kpn I酶切Southern blot; WT: 野生型对照Xba I酶切Southern blot; M: λDNA/Hind III marker。

Fig. 5 Southern blot figure of marker free transgenic soybean of T5 generation 1: Southern blot of transgenic soybean digested by Xba I; 2: Southern blot of transgenic soybean digested by Kpn I; WT: Southern blot of wild type controls digested by Xba I; M: λDNA/Hind III marker.