基于Cre/loxP系统的无筛选标记转耐低磷转录因子GmPTF1大豆种质创制与分析

张小芳,董秋平,乔潇,乔亚科,王冰冰,张锴,李桂兰

Creation and analysis of marker free transgenic soybean germplasm with low phosphate tolerance transcription factor GmPTF1 based on Cre/loxP system

Xiao-Fang ZHANG,Qiu-Ping DONG,Xiao QIAO,Ya-Ke QIAO,Bing-Bing WANG,Kai ZHANG,Gui-Lan LI

图6 无筛选标记转基因大豆GmPTF1基因RT-PCR H: 空白对照; WT: 野生型对照; 1~7: 转基因植株; P: 质粒阳性对照; M: 100 bp ladder DNA marker。

Fig. 6 GmPTF1 RT-PCR of marker free transgenic soybean H: blank control; WT: wild type control; 1-7: transgenic soybean; P: plasmid positive control; M: 100 bp DNA marker ladder.