基于Cre/loxP系统的无筛选标记转耐低磷转录因子GmPTF1大豆种质创制与分析

张小芳,董秋平,乔潇,乔亚科,王冰冰,张锴,李桂兰

Creation and analysis of marker free transgenic soybean germplasm with low phosphate tolerance transcription factor GmPTF1 based on Cre/loxP system

Xiao-Fang ZHANG,Qiu-Ping DONG,Xiao QIAO,Ya-Ke QIAO,Bing-Bing WANG,Kai ZHANG,Gui-Lan LI

图8 无筛选标记转基因大豆不同器官中GmPTF1蛋白的Western杂交结果 1: 野生型对照; 2: 转基因材料; A: GmPTF基因表达蛋白; B: Actin基因表达蛋白。

Fig. 8 Western blot results of GmPTF1 in different organs of marker free transgenic soybean 1: wild type controls; 2: transgenic plants; A: GmPTF1 protein; B: Actin protein.