小麦芒长抑制基因B2的精细定位与候选基因分析

金迪,王冬至,王焕雪,李润枝,陈树林,阳文龙,张爱民,刘冬成,詹克慧

Fine mapping and candidate gene analysis of awn inhibiting gene B2 in common wheat

Di JIN,Dong-Zhi WANG,Huan-Xue WANG,Run-Zhi LI,Shu-Lin CHEN,Wen-Long YANG,Ai-Min ZHANG,Dong-Cheng LIU,Ke-Hui ZHAN

表1 本研究所用到的引物

Table 1 Primers used in this study

引物名称 Primer ID 正向引物序列 Forward sequence (5'-3') 反向引物序列 Reverse sequence (5'-3') 内切酶 Restriction enzyme 片段大小 Size (bp) B2精细定位 Markers for mapping B2 Xgwm88 * CACTACAACTATGCGCTCGC TCCATTGGCTTCTCTCTCAA — 120 SSR43 ACTATCACAAGCCGGAGTAA AGTGGTTAAATCGCCACTAA — 172 SSR122 CATGTTACCACCAAAGGATT CTATGTGGCTGACCGTTACT — 205 EST-499300LC TCCAAAGAATCCTGGAGGTG TGTTCTGACCATCCCACGTA Pvu II 393/270 dCAPS-18 GACCACCCGACACCGCCGCTGCA GCTCATCTCCAATAGCTCGC Pst I 204/184 dCAPS-19 CAAAACTTCTTGGGAGAATATAC CCCTGACATCGTCTCGAACT Mlu I 192/172 dCAPS-4 GTAAGCAGCGAGGTTAGCACTGC CTGGTGCTCATGAGTTGTGG Pst I 296/276 EST-263300 CGTGTGCTTAAATGCTCCATGG GCTGACTTATGCTGCTTTACTTTCTAA Taq I 521/472 CAPS-12 ATTGATGCAAAGGAACCAGG GAGTTCCATGGCCTCATTGT Eco RI 308/236 CAPS-14 GGAAGGTCCACTTTGCATGT CACCACAGGGACGAAATTCT Bam HI 281/265 dCAPS-39 GTTTTCTTTGTAGAAGTCCCATA AAACACCTGAAAATCGTGGC Nde I 185/165 EST-265400 CCATCAGTCCAACCATGACTTGT TAACATAAGCGTCGCTGTGGC Pst I 246/(111+135) CAPS-19 TGTCCTTGAAGGGGGTAGTG TCCTGCATTATGCCACAAAA Nde I 483/(217+266) WABM232658 * AAGTTCGCCTCTTCACCAGT TCTGCCCCTACATCTGTTGC — 130 SSR74 CATAATCACAATTCATCGGA TTCATACCTGACCCATCTTC — 214 SSR116 AGAATCAGTTTTCAGCCAGA AACTATCCCGTATACTTGCG — 168 WABM214868 * GGGTGCCTGAACATTGATGC CCCCAAGTGCTGTCGTGTAT — 126 候选基因克隆 Gene cloning primers 6B01G262600 AGCGAAGCAGTCAGTACTCAGT CTTGCATGGATAATCGAAACATAGGA — 1041 6B01G263300 CGTGTGCTTAAATGCTCCATGG GCTGACTTATGCTGCTTTACTTTCTAA — 1110 6B01G264600 GCCCATTTTTGCAGTCATGACATCA CGGGATGAAAACCTGCTCTTC — 1760 6B01G265400 CCATCAGTCCAACCATGACTTGT TAACATAAGCGTCGCTGTGGC — 1071 6B01G504500LC CACACACACACACACCAAAACTC GGCATACTGCGAACAACCCA — 985 6B01G505500LC AGCAGCTGGGTGAAGTTAACTA TTCCAGCCCCCTTTTACAAGAT — 1437