利用CRISPR/Cas9技术创制大豆高油酸突变系

侯智红,吴艳,程群,董利东,芦思佳,南海洋,甘卓然,刘宝辉

Creation of high oleic acid soybean mutation plants by CRISPR/Cas9

Zhi-Hong HOU,Yan WU,Qun CHENG,Li-Dong DONG,Si-Jia LU,Hai-Yang NAN,Zhuo-Ran GAN,Bao-Hui LIU

表1 引物序列

Table 1 Primer sequence

引物名称 Primer name 寡核苷酸序列 Oligonucleotide sequence (5'-3') FAD-AtU3d-T1-F1 gtcACAAAGCCACCATTCACTGT FAD-AtU3d-T1-R1 aaacACAGTGAATGGTGGCTTTG FAD-AtU3b-T2-F2 gtcAACCAAAATCCAAAGTTGCA FAD-AtU3b-T2-R2 aaacTGCAACTTTGGATTTTGGT FAD-AtU6-1-T3-F3 attGTTTGGCTGCTATGTGTTTA FAD-AtU6-1-T3-R3 aaacTAAACACATAGCAGCCAAA FAD2-1A-CasF-Test GAGGGATTGTAGTTCTGTTG FAD2-1A-CasR-Test CTATGGCCCATTGGTTGCTC U-F CTCCGTTTTACCTGTGGAATCG gRNA-R CGGAGGAAAATTCCATCCAC B1’ TTCAGAggtctcTctcgCACTGGAATCGGCAGCAAAGG B2 AGCGTGggtctcGtcagGGTCCATCCACTCCAAGCTC B2’ TTCAGAggtctcTctgaCACTGGAATCGGCAGCAAAGG B3 AGCGTGggtctcGtcttGGTCCATCCACTCCAAGCTC B3’ TTCAGAggtctcTaagaCACTGGAATCGGCAGCAAAGG BL AGCGTGggtctcGaccgGGTCCATCCACTCCAAGCTC Bar-F TGCCAGTTCCCGTGCTTGAA Bar-R CTGCACCATCGTCAACCACTA