利用CRISPR/Cas9技术创制大豆高油酸突变系

侯智红,吴艳,程群,董利东,芦思佳,南海洋,甘卓然,刘宝辉

Creation of high oleic acid soybean mutation plants by CRISPR/Cas9

Zhi-Hong HOU,Yan WU,Qun CHENG,Li-Dong DONG,Si-Jia LU,Hai-Yang NAN,Zhuo-Ran GAN,Bao-Hui LIU

图4 重组质粒鉴定 A: 菌落PCR检测电泳; M: DNA marker (DM2000); 1: H2O空白对照; 2: pYLCRISPR/Cas9-DB质粒; 3~7: FAD2-1A基因敲除阳性单菌落。B: Asc I酶切鉴定pYLCRISPR/Cas9-FAD2-1A-gRNA载体; M: 1 kb DNA ladder marker; 1: pYLCRISPR/Cas9-FAD2-1A- gRNA。

Fig. 4 Identification of recombinant plasmids A: Electrophoresis of PCR detection of clony; M: DNA marker (DM2000); 1: H2O blank control; 2: pYLCRISPR/Cas9-DB plasmid; 3-7: FAD2-1A gene knockout positive single clones. B: Identification of the pYLCRISPR/Cas9-FAD2-1A-gRNA plasmid digested with Asc I; M: 1 kb DNA ladder marker; 1: pYLCRISPR/Cas9- FAD2-1A-gRNA.