利用CRISPR/Cas9技术创制大豆高油酸突变系

侯智红,吴艳,程群,董利东,芦思佳,南海洋,甘卓然,刘宝辉

Creation of high oleic acid soybean mutation plants by CRISPR/Cas9

Zhi-Hong HOU,Yan WU,Qun CHENG,Li-Dong DONG,Si-Jia LU,Hai-Yang NAN,Zhuo-Ran GAN,Bao-Hui LIU

图5 转化过程及叶片涂抹草铵膦筛选结果 A: 农杆菌介导的子叶节法转化大豆; 1: 无菌苗的萌发; 2: 共培养; 3: 丛生芽的诱导; 4: 丛生芽的伸长; 5: 诱导生根; 6: 移栽土里。 B: T0转基因大豆植株草铵膦涂抹鉴定; a: 阳性转基因植株; b: 阴性转基因植株。

Fig. 5 Conversion process and the results of leaf painting A: transformation soybean cotyledonine by agrobacterium; 1: germination of sterile seedlings; 2: co-cultivation; 3: shoot induction; 4: shoot elongation; 5: rooting of the resistant shoot; 6: seedling acclimatization. B: identification of the herbicide resistance of the leaves of T0 transgenic soybean plants using glufosinate; a: positive transgenic plant; b: negative transgenic plant.